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ERG Rabbit mAb (A22748)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Rat

ABclonal:Western blot - ERG Rabbit mAb (A22748)

Western blot analysis of lysates from HEL cells, using ERG Rabbit mAb (A22748) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Immunohistochemistry - ERG Rabbit mAb (A22748)

Immunohistochemistry analysis of paraffin-embedded Human appendix using ERG Rabbit mAb (A22748) at dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ERG Rabbit mAb (A22748)

Immunohistochemistry analysis of paraffin-embedded Human endometrium cancer using ERG Rabbit mAb (A22748) at dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ERG Rabbit mAb (A22748)

Immunohistochemistry analysis of paraffin-embedded Human tonsil using ERG Rabbit mAb (A22748) at dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

Overview

Product nameERG Rabbit mAb
Catalog No.A22748
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC58302
This gene encodes a member of the erythroblast transformation-specific (ETS) family of transcriptions factors. All members of this family are key regulators of embryonic development, cell proliferation, differentiation, angiogenesis, inflammation, and apoptosis. The protein encoded by this gene is mainly expressed in the nucleus. It contains an ETS DNA-binding domain and a PNT (pointed) domain which is implicated in the self-association of chimeric oncoproteins. This protein is required for platelet adhesion to the subendothelium, inducing vascular cell remodeling. It also regulates hematopoesis, and the differentiation and maturation of megakaryocytic cells. This gene is involved in chromosomal translocations, resulting in different fusion gene products, such as TMPSSR2-ERG and NDRG1-ERG in prostate cancer, EWS-ERG in Ewing's sarcoma and FUS-ERG in acute myeloid leukemia. More than two dozens of transcript variants generated from combinatorial usage of three alternative promoters and multiple alternative splicing events have been reported, but the full-length nature of many of these variants has not been determined.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 379-479 of human ERG. (NP_891548.1).
SequenceTKVHGKRYAYKFDFHGIAQALQPHPPESSLYKYPSDLPYMGSYHAHPQKMNFVAPHPPALPVTSSSFFAAPNPYWNSPTGGIYPNTRLPTSHMPSHLGTYY
Gene ID
Swiss Prot
Synonymsp55; erg-3; ERG
Calculated MW54kDa
Observed MW54kDa
ReactivityHuman, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMCF7 cells
Cellular locationNucleus.

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Lot number

ABclonal:Western blot - ERG Rabbit mAb (A22748)}

Western blot - ERG Rabbit mAb (A22748)

Western blot analysis of lysates from HEL cells, using ERG Rabbit mAb (A22748) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
ABclonal:Immunohistochemistry - ERG Rabbit mAb (A22748)}

Immunohistochemistry - ERG Rabbit mAb (A22748)

Immunohistochemistry analysis of paraffin-embedded Human appendix using ERG Rabbit mAb (A22748) at dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ERG Rabbit mAb (A22748)}

Immunohistochemistry - ERG Rabbit mAb (A22748)

Immunohistochemistry analysis of paraffin-embedded Human endometrium cancer using ERG Rabbit mAb (A22748) at dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ERG Rabbit mAb (A22748)}

Immunohistochemistry - ERG Rabbit mAb (A22748)

Immunohistochemistry analysis of paraffin-embedded Human tonsil using ERG Rabbit mAb (A22748) at dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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