제품 > 항체 > 단일클론항체(mAb)

ABflo® 488 Rabbit anti-Human IL9 mAb (A24306)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Confocal imaging of 293T cells transfected with IL9 using ABflo® 488 Rabbit anti-Human IL9 mAb (A24306, dilution 1:50). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Human IL9 mAb (A24306)

Flow cytometry:1X10^6 293T cells (negative control, left) and 293T(Transfection, right) cells were intracellularly-stained with ABflo® 488 Rabbit anti-Human IL9 mAb(A24306, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry:1X10^6 293T(Transfection) cells were intracellularly-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human IL9 mAb(A24306, 5 μl/Test, right).

Overview

Product name	ABflo® 488 Rabbit anti-Human IL9 mAb
Catalog No.	A24306
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC62039-ABflo488

Background

The protein encoded by this gene is a cytokine that acts as a regulator of a variety of hematopoietic cells. This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. The gene encoding this cytokine has been identified as a candidate gene for asthma. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 19-144 of human IL9(NP_000581.1).
Sequence	QGCPTLAGILDINFLINKMQEDPASKCHCSANVTSCLCLGIPSDNCTRPCFSERLSQMTNTTMQTRYPLIFSRVKKSVEVLKNNKCPYFSCEQPCNQTTAGNALTFLKSLLEIFQKEKMRGMRGKI
Gene ID
Swiss Prot
Synonyms	IL9; HP40; IL-9; P40; interleukin-9
Calculated MW	15kDa
Observed MW

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	IF/ICC 1:50 - 1:200 FC (intra) 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application	Immunofluorescence
Positive samples
Cellular location	Secreted.

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

ABflo® 488 Rabbit anti-Human IL9 mAb images

Immunofluorescence - ABflo® 488 Rabbit anti-Human IL9 mAb (A24306)

Confocal imaging of 293T cells transfected with IL9 using ABflo® 488 Rabbit anti-Human IL9 mAb (A24306, dilution 1:50). DAPI was used for nuclear staining (Blue). Objective: 100x.

Flow CytoMetry - ABflo® 488 Rabbit anti-Human IL9 mAb (A24306)

* For research use only. Not for therapeutic or diagnostic purposes.