제품 > 항체 > 단일클론항체(mAb)

ABflo® 488 Rabbit anti-Human ITGA1/CD49a mAb (A22691)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Flow cytometry:1X10^6 K-562 cells (negative control, Left) and SH-SY5Y cells (Right) were surface-stained with ABflo® 488 Rabbit anti-Human ITGA1/CD49a mAb (A22691, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry:1X10^6 SH-SY5Y cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Human ITGA1/CD49a mAb (A22691, 5 μl/Test, right).

Overview

Product name	ABflo® 488 Rabbit anti-Human ITGA1/CD49a mAb
Catalog No.	A22691
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC58076-ABf488

Background

This gene encodes the alpha 1 subunit of integrin receptors. This protein heterodimerizes with the beta 1 subunit to form a cell-surface receptor for collagen and laminin. The heterodimeric receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. The alpha 1 subunit contains an inserted (I) von Willebrand factor type I domain which is thought to be involved in collagen binding.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 29-1141 of human ITGA1/CD49a ( NP_852478.1).
Sequence	FNVDVKNSMT FSGPVEDMFG YTVQQYENEE GKWVLIGSPL VGQPKNRTGD VYKCPVGRGE SLPCVKLDLP VNTSIPNVTE VKENMTFGST LVTNPNGGFL ACGPLYAYRC GHLHYTTGIC SDVSPTFQVV NSIAPVQECS TQLDIVIVLD GSNSIYPWDS VTAFLNDLLE RMDIGPKQTQ VGIVQYGENV THEFNLNKYS STEEVLVAAK KIVQRGGRQT MTALGIDTAR KEAFTEARGA RRGVKKVMVI VTDGESHDNH RLKKVIQDCE DENIQRFSIA ILGSYNRGNL STEKFVEEIK SIASEPTEKH FFNVSDELAL VTIVKTLGER IFALEATADQ SAASFEMEMS QTGFSAHYSQ DWVMLGAVGA YDWNGTVVMQ KASQIIIPRN TTFNVESTKK NEPLASYLGY TVNSATASSG DVLYIAGQPR YNHTGQVIIY RMEDGNIKIL QTLSGEQIGS YFGSILTTTD IDKDSNTDIL LVGAPMYMGT EKEEQGKVYV YALNQTRFEY QMSLEPIKQT CCSSRQHNSC TTENKNEPCG ARFGTAIAAV KDLNLDGFND IVIGAPLEDD HGGAVYIYHG SGKTIRKEYA QRIPSGGDGK TLKFFGQSIH GEMDLNGDGL TDVTIGGLGG AALFWSRDVA VVKVTMNFEP NKVNIQKKNC HMEGKETVCI NATVCFDVKL KSKEDTIYEA DLQYRVTLDS LRQISRSFFS GTQERKVQRN ITVRKSECTK HSFYMLDKHD FQDSVRITLD FNLTDPENGP VLDDSLPNSV HEYIPFAKDC GNKEKCISDL SLHVATTEKD LLIVRSQNDK FNVSLTVKNT KDSAYNTRTI VHYSPNLVFS GIEAIQKDSC ESNHNITCKV GYPFLRRGEM VTFKILFQFN TSYLMENVTI YLSATSDSEE PPETLSDNVV NISIPVKYEV GLQFYSSASE YHISIAANET VPEVINSTED IGNEINIFYL IRKSGSFPMP ELKLSISFPN MTSNGYPVLY PTGLSSSENA NCRPHIFEDP FSINSGKKMT TSTDHLKRGT ILDCNTCKFA TITCNLTSSD ISQVNVSLIL WKPTFIKSYF SSLNLTIRGE LRSENASLVL SSSNQKRELA IQISKDGLPG RVP
Gene ID
Swiss Prot
Synonyms	VLA1; CD49a
Calculated MW	131kDa
Observed MW

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	cell surface, external side of plasma membrane, extracellular exosome, focal adhesion, plasma membrane