제품 > 항체 > 단일클론항체(mAb)

ABflo® 488 Rabbit anti-Mouse CD22 mAb (A24627)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse

Confocal imaging of paraffin-embedded Mouse spleen tissue using ABflo® 488 Rabbit anti-Mouse CD22 mAb (A24627, dilution 1:200). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Mouse CD22 mAb (A24627)

Flow cytometry: 1X10^6 NIH/3T3 cells (negative control, left) and C57BL/6 splenocytes (right) were surface-stained with ABflo® 488 Rabbit anti-Mouse CD22 mAb (A24627, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 C57BL/6 splenocytes were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Mouse CD22 mAb (A24627, 5 μl/Test, right).

Overview

Product name	ABflo® 488 Rabbit anti-Mouse CD22 mAb
Catalog No.	A24627
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC62722-ABflo488

Background

Enables CD4 receptor binding activity; protein phosphatase binding activity; and sialic acid binding activity. Involved in several processes, including negative regulation of calcium-mediated signaling; negative regulation of immunoglobulin production; and regulation of B cell proliferation. Located in external side of plasma membrane and neuronal cell body membrane. Is integral component of plasma membrane. Is expressed in several structures, including alimentary system; brain; hemolymphoid system gland; liver and biliary system; and reproductive system. Orthologous to human CD22 (CD22 molecule).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 22-151 of mouse CD22 (NP_033975.3).
Sequence	SANDWTVDHPQTLFAWEGACIRIPCKYKTPLPKARLDNILLFQNYEFDKATKKFTGTVLYNATKTEKDPESELYLSKQGRVTFLGNRIDNCTLKIHPIRANDSGNLGLRMTAGTERWMEPIHLNVSEKPF
Gene ID
Swiss Prot
Synonyms	Lyb8; Lyb-8; A530093D23
Calculated MW	89kDa/93kDa/97kDa
Observed MW	Refer to figures

Applications

Reactivity	Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	IF/ICC 1:50 - 1:200 FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application	Immunofluorescence Flow Cytometry
Positive samples
Cellular location	Cell membrane , Single-pass type I membrane protein