제품 > 항체 > 단일클론항체(mAb)

ABflo® 488 Rabbit anti-Mouse CD6 mAb (A25563)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse

Flow cytometry: 1X10^6 NIH/3T3 cells (negative control, left) and C57BL/6 mouse Splenocytes (right) were surface-stained with ABflo® 488 Rabbit anti-Mouse CD6 mAb (A25563, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 C57BL/6 mouse Splenocytes were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Mouse CD6 mAb (A25563, 5 μl/Test, right).

Overview

Product name	ABflo® 488 Rabbit anti-Mouse CD6 mAb
Catalog No.	A25563
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC66616-ABflo488

Background

Predicted to enable several functions, including identical protein binding activity; lipopolysaccharide binding activity; and lipoteichoic acid binding activity. Predicted to be involved in several processes, including heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules; lipopolysaccharide-mediated signaling pathway; and positive regulation of macromolecule metabolic process. Predicted to act upstream of or within cell adhesion. Predicted to be located in immunological synapse. Predicted to be spanning component of plasma membrane. Predicted to be part of T cell receptor complex. Predicted to be active in external side of plasma membrane. Orthologous to human CD6 (CD6 molecule).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 20-155 of mouse CD6(NP_033982.3).
Sequence	SPAPSGQHKNGTIPNMTLDLEERLGIRLVNGSSRCSGSVKVLLESWEPVCAAHWNRAATEAVCKALNCGDSGKVTYLMPPTSELPPGATSGNTSSAGNTTWARAPTERCRGANWQFCKVQDQECSSDRRLVWVTCA
Gene ID
Swiss Prot
Synonyms	Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Calculated MW	64kDa/68kDa/72kDa
Observed MW

Applications

Reactivity	Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Cell membrane