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ABflo® 488 Rabbit anti-Mouse CXCR2/IL-8RB mAb (A26757)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Mouse

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Mouse CXCR2/IL-8RB mAb (A26757)

Flow cytometry: 1X10^6 293T cells (negative control, left) and 293T (Transfection, right) cells were surface-stained with ABflo® 488 Rabbit anti-Mouse CXCR2/IL-8RB mAb (A26757, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Mouse CXCR2/IL-8RB mAb (A26757)

Flow cytometry: 1X10^6 293T (Transfection) cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Mouse CXCR2/IL-8RB mAb (A26757, 5 μl/Test, right).

ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Mouse CXCR2/IL-8RB mAb (A26757)

Flow cytometry: 1X10^6 RAW264.7 cells (negative control, left) and C57BL/6 mouse bone marrow cells (right) were surface-stained with ABflo® 488 Rabbit anti-Mouse CXCR2/IL-8RB mAb (A26757, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Overview

Product nameABflo® 488 Rabbit anti-Mouse CXCR2/IL-8RB mAb
Catalog No.A26757
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC65373-ABflo488
Enables chemokine receptor activity. Acts upstream of or within negative regulation of apoptotic process. Predicted to be located in several cellular components, including cell surface; mast cell granule; and mitotic spindle. Predicted to be active in external side of plasma membrane. Is expressed in several structures, including alimentary system; central nervous system; embryo mesenchyme; epithelium; and heart. Human ortholog(s) of this gene implicated in IgA glomerulonephritis; acute pyelonephritis; end stage renal disease; and urinary bladder cancer. Orthologous to human CXCR2 (C-X-C motif chemokine receptor 2).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-44 of mouse CXCR2/IL-8RB (NP_034039.1).
SequenceMGEFKVDKFNIEDFFSGDLDIFNYSSGMPSILPDAVPCHSENLE
Gene ID
Swiss Prot
SynonymsCD128; IL8RA; Il8rb; CDw128; Cmkar2; Gpcr16; IL-8Rh; IL-8rb; mIL-8RH
Calculated MW40kDa
Observed MW
ReactivityMouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationCell membrane.

Documents

Certificate of Compliance

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Lot number

    ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Mouse CXCR2/IL-8RB mAb (A26757)}

    Flow CytoMetry - ABflo® 488 Rabbit anti-Mouse CXCR2/IL-8RB mAb (A26757)

    Flow cytometry: 1X10^6 293T cells (negative control, left) and 293T (Transfection, right) cells were surface-stained with ABflo® 488 Rabbit anti-Mouse CXCR2/IL-8RB mAb (A26757, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
    ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Mouse CXCR2/IL-8RB mAb (A26757)}

    Flow CytoMetry - ABflo® 488 Rabbit anti-Mouse CXCR2/IL-8RB mAb (A26757)

    Flow cytometry: 1X10^6 293T (Transfection) cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Mouse CXCR2/IL-8RB mAb (A26757, 5 μl/Test, right).
    ABclonal:Flow CytoMetry - ABflo® 488 Rabbit anti-Mouse CXCR2/IL-8RB mAb (A26757)}

    Flow CytoMetry - ABflo® 488 Rabbit anti-Mouse CXCR2/IL-8RB mAb (A26757)

    Flow cytometry: 1X10^6 RAW264.7 cells (negative control, left) and C57BL/6 mouse bone marrow cells (right) were surface-stained with ABflo® 488 Rabbit anti-Mouse CXCR2/IL-8RB mAb (A26757, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

    * For research use only. Not for therapeutic or diagnostic purposes.

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