제품 > 항체 > 단일클론항체(mAb)

ABflo® 488 Rabbit anti-Mouse IL-33R/ST2 mAb (A26400)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse

Flow cytometry: 1X10^6 C2C12 cells (negative control, left) and P815 cells (right) were surface-stained with ABflo® 488 Rabbit anti-Mouse IL-33R/ST2 mAb (A26400, 5 μl/Test, orange line) or ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 P815 cells were surface-stained with ABflo® 488 Rabbit IgG isotype control (A22069, 5 μl/Test, left) or ABflo® 488 Rabbit anti-Mouse IL-33R/ST2 mAb (A26400, 5 μl/Test, right).

Overview

Product name	ABflo® 488 Rabbit anti-Mouse IL-33R/ST2 mAb
Catalog No.	A26400
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC67060-ABflo488

Background

Enables interleukin-33 binding activity and interleukin-33 receptor activity. Involved in several processes, including negative regulation of T-helper 1 type immune response; positive regulation of macrophage activation; and regulation of cytokine production. Acts upstream of or within negative regulation of I-kappaB kinase/NF-kappaB signaling. Located in external side of plasma membrane and extracellular matrix. Is expressed in several structures, including immune system; musculoskeletal system; salivary gland; skin; and visual system. Human ortholog(s) of this gene implicated in asthma and coronary artery disease. Orthologous to human IL1RL1 (interleukin 1 receptor like 1).

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 27-332 of mouse IL-33R /ST2 (NP_001020773.1).
Sequence	SKSSWGLENEALIVRCPQRGRSTYPVEWYYSDTNESIPTQKRNRIFVSRDRLKFLPARVEDSGIYACVIRSPNLNKTGYLNVTIHKKPPSCNIPDYLMYSTVRGSDKNFKITCPTIDLYNWTAPVQWFKNCKALQEPRFRAHRSYLFIDNVTHDDEGDYTCQFTHAENGTNYIVTATRSFTVEEKGFSMFPVITNPPYNHTMEVEIGKPASIACSACFGKGSHFLADVLWQINKTVVGNFGEARIQEEEGRNESSSNDMDCLTSVLRITGVTEKDLSLEYDCLALNLHGMIRHTIRLRRKQPIDHR
Gene ID
Swiss Prot
Synonyms	T1; St2; DER4; Ly84; ST2L; Fit-1; T1/ST2; St2-rs1
Calculated MW	38kDa/65kDa
Observed MW	Refer to figures

Applications

Reactivity	Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Cell membrane, Single-pass type I membrane protein, Secreted.