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ABflo® 594 Rabbit anti-Dog CD3G mAb (A23352)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Dog

ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Dog CD3G mAb (A23352)

Flow cytometry:1X10^6 293F cells (negative control, Left) and 293F(Transfection, right) cells were surface-stained with ABflo® 594 Rabbit anti-Dog CD3G mAb(A23352, 5 μl/Test, orange line) or ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Dog CD3G mAb (A23352)

Flow cytometry:1X10^6 293F(Transfection) cells were surface-stained with ABflo® 594 Rabbit IgG isotype control (5 μl/Test, left) or ABflo® 594 Rabbit anti-Dog CD3G mAb(A23352, 5 μl/Test, right).

Overview

Product nameABflo® 594 Rabbit anti-Dog CD3G mAb
Catalog No.A23352
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC57945-ABf594
The protein encoded by this gene is the CD3-gamma polypeptide, which together with CD3-epsilon, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. Defects in this gene are associated with T cell immunodeficiency.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 23-116 of dog CD3G(XP_005619768.1)
SequenceQKEQGFPMIKVDGNREDGSVLLICDSQSKDIKWFEDGKERTLPKKDKKTLDLGSSMKDPQGIYQCQVAKNISKPLQVYYRMCQNCIELNAGTII
Gene ID
Swiss Prot
Synonyms
Calculated MW20kDa
Observed MW
ReactivityDog
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationPlasma membrane.

Documents

Certificate of Compliance

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Lot number

    ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Dog CD3G mAb (A23352)}

    Flow CytoMetry - ABflo® 594 Rabbit anti-Dog CD3G mAb (A23352)

    Flow cytometry:1X10^6 293F cells (negative control, Left) and 293F(Transfection, right) cells were surface-stained with ABflo® 594 Rabbit anti-Dog CD3G mAb(A23352, 5 μl/Test, orange line) or ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
    ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Dog CD3G mAb (A23352)}

    Flow CytoMetry - ABflo® 594 Rabbit anti-Dog CD3G mAb (A23352)

    Flow cytometry:1X10^6 293F(Transfection) cells were surface-stained with ABflo® 594 Rabbit IgG isotype control (5 μl/Test, left) or ABflo® 594 Rabbit anti-Dog CD3G mAb(A23352, 5 μl/Test, right).

    * For research use only. Not for therapeutic or diagnostic purposes.