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ABflo® 594 Rabbit anti-Human CD112/Nectin-2 mAb (A24329)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Human CD112/Nectin-2 mAb (A24329)

Flow cytometry:1X10^6 MOLT-4 cells (negative control, Left) and MCF-7 cells (Right) were surface-stained with ABflo® 594 Rabbit anti-Human CD112/Nectin-2 mAb(A24329, 5 μl/Test, orange line) or ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Human CD112/Nectin-2 mAb (A24329)

Flow cytometry:1X10^6 MCF-7 cells were surface-stained with ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, left) or ABflo® 594 Rabbit anti-Human CD112/Nectin-2 mAb(A24329, 5 μl/Test, right).

Overview

Product nameABflo® 594 Rabbit anti-Human CD112/Nectin-2 mAb
Catalog No.A24329
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC62371-ABflo594
This gene encodes a single-pass type I membrane glycoprotein with two Ig-like C2-type domains and an Ig-like V-type domain. This protein is one of the plasma membrane components of adherens junctions. It also serves as an entry for certain mutant strains of herpes simplex virus and pseudorabies virus, and it is involved in cell to cell spreading of these viruses. Variations in this gene have been associated with differences in the severity of multiple sclerosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
ImmunogenRecombinant Protein corresponding to a sequence within amino acids 32-158 of human CD112/Nectin-2 (NP_001036189.1).
SequenceQDVRVQVLPEVRGQLGGTVELPCHLLPPVPGLYISLVTWQRPDAPANHQNVAAFHPKMGPSFPSPKPGSERLSFVSAKQSTGQDTEAELQDATLALHGLTVEDEGNYTCEFATFPKGSVRGMTWLRV
Gene ID
Swiss Prot
SynonymsNECTIN2; CD112; HVEB; PRR2; PVRL2; PVRR2; nectin-2
Calculated MW51kDa/57kDa
Observed MWRefer to figures
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationCell membrane, Single-pass type I membrane protein

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ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Human CD112/Nectin-2 mAb (A24329)}

Flow CytoMetry - ABflo® 594 Rabbit anti-Human CD112/Nectin-2 mAb (A24329)

Flow cytometry:1X10^6 MOLT-4 cells (negative control, Left) and MCF-7 cells (Right) were surface-stained with ABflo® 594 Rabbit anti-Human CD112/Nectin-2 mAb(A24329, 5 μl/Test, orange line) or ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Human CD112/Nectin-2 mAb (A24329)}

Flow CytoMetry - ABflo® 594 Rabbit anti-Human CD112/Nectin-2 mAb (A24329)

Flow cytometry:1X10^6 MCF-7 cells were surface-stained with ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, left) or ABflo® 594 Rabbit anti-Human CD112/Nectin-2 mAb(A24329, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.

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