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ABflo® 594 Rabbit anti-Human LILRA2/CD85h mAb (A25568)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Immunofluorescence - ABflo® 594 Rabbit anti-Human LILRA2/CD85h mAb (A25568)

Confocal imaging of 293T cells transfected with LILRA2/CD85h using ABflo® 594 Rabbit anti-Human LILRA2/CD85h mAb (A25568, dilution 1:200). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Human LILRA2/CD85h mAb (A25568)

Flow cytometry: 1X10^6 293T cells (negative control, left) and 293T (Transfection, right) cells were surface-stained with ABflo® 594 Rabbit anti-Human LILRA2/CD85h mAb (A25568, 5 μl/Test, orange line) or ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Human LILRA2/CD85h mAb (A25568)

Flow cytometry: 1X10^6 293T (Transfection) cells were surface-stained with ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, left) or ABflo® 594 Rabbit anti-Human LILRA2/CD85h mAb (A25568, 5 μl/Test, right).

Overview

Product nameABflo® 594 Rabbit anti-Human LILRA2/CD85h mAb
Catalog No.A25568
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC65539-ABflo594
This gene encodes a member of a family of immunoreceptors that are expressed predominantly on monocytes and B cells, and at lower levels on dendritic cells and natural killer cells. The encoded protein is an activating receptor that inhibits dendritic cell differentiation and antigen presentation and suppresses innate immune response. Alternatively spliced transcript variants encoding different isoforms have been found. This gene is located in a cluster of related genes on chromosome 19 and there is a pseudogene for this gene on chromosome 3.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 24-449 of human LILRA2/CD85h (NP_001124389.2).
SequenceGHLPKPTLWAEPGSVIIQGSPVTLRCQGSLQAEEYHLYRENKSASWVRRIQEPGKNGQFPIPSITWEHAGRYHCQYYSHNHSSEYSDPLELVVTGAYSKPTLSALPSPVVTSGGNVTLQCVSQVAFDGFILCKEGEDEHPQRLNSHSHARGWSWAIFSVGPVSPSRRWSYRCYAYDSNSPYVWSLPSDLLELLVPGVSKKPSLSVQPGPMVAPGESLTLQCVSDVGYDRFVLYKEGERDFLQRPGWQPQAGLSQANFTLGPVSPSHGGQYRCYSAHNLSSEWSAPSDPLDILITGQFYDRPSLSVQPVPTVAPGKNVTLLCQSRGQFHTFLLTKEGAGHPPLHLRSEHQAQQNQAEFRMGPVTSAHVGTYRCYSSLSSNPYLLSLPSDPLELVVSEAAETLSPSQNKTDSTTTSLGQHPQDYTVEN
Gene ID
Swiss Prot
SynonymsILT1; LIR7; CD85H; LIR-7
Calculated MW53kDa/51kDa/48kDa/47kDa
Observed MW
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IF/ICC 1:50 - 1:200
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key applicationImmunofluorescence    Flow Cytometry    
Positive samples
Cellular locationMembrane, Secreted, Single-pass type I membrane protein.

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ABclonal:Immunofluorescence - ABflo® 594 Rabbit anti-Human LILRA2/CD85h mAb (A25568)}

Immunofluorescence - ABflo® 594 Rabbit anti-Human LILRA2/CD85h mAb (A25568)

Confocal imaging of 293T cells transfected with LILRA2/CD85h using ABflo® 594 Rabbit anti-Human LILRA2/CD85h mAb (A25568, dilution 1:200). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Human LILRA2/CD85h mAb (A25568)}

Flow CytoMetry - ABflo® 594 Rabbit anti-Human LILRA2/CD85h mAb (A25568)

Flow cytometry: 1X10^6 293T cells (negative control, left) and 293T (Transfection, right) cells were surface-stained with ABflo® 594 Rabbit anti-Human LILRA2/CD85h mAb (A25568, 5 μl/Test, orange line) or ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
ABclonal:Flow CytoMetry - ABflo® 594 Rabbit anti-Human LILRA2/CD85h mAb (A25568)}

Flow CytoMetry - ABflo® 594 Rabbit anti-Human LILRA2/CD85h mAb (A25568)

Flow cytometry: 1X10^6 293T (Transfection) cells were surface-stained with ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, left) or ABflo® 594 Rabbit anti-Human LILRA2/CD85h mAb (A25568, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.

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