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ABflo® 594 Rabbit anti-Human Phospho-PI3KP85α-Y467+P85β-Y464+P55γ-Y199 mAb (AP1489)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Flow cytometry: 1X10^6 293T cells (negative control, left) and 293T (Transfection, right) cells were intracellularly-stained with ABflo® 594 Rabbit anti-Human Phospho-PI3KP85α-Y467+P85β-Y464+P55γ-Y199 mAb (AP1489, 5 μl/Test, orange line) or ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 293T (Transfection) cells were intracellularly-stained with ABflo® 594 Rabbit IgG isotype control (A23821, 5 μl/Test, left) or ABflo® 594 Rabbit anti-Human Phospho-PI3KP85α-Y467+P85β-Y464+P55γ-Y199 mAb (AP1489, 5 μl/Test, right).

Overview

Product name	ABflo® 594 Rabbit anti-Human Phospho-PI3KP85α-Y467+P85β-Y464+P55γ-Y199 mAb
Catalog No.	AP1489
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC65199-ABflo594

Background

Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]

Immunogen information

Immunogen	A synthetic phosphorylated peptide around Y467 of human PI3KP85α (NP_852664.1).
Sequence	RLYEEY
Gene ID
Swiss Prot
Synonyms	PIK3R1/PIK3R2/PIK3R3
Calculated MW	42-54kDa/81-84kDa
Observed MW

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC (intra) 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application
Positive samples
Cellular location	Golgi network, Cytoplasm, Cytosol, Nucleus, Perinuclear region of cytoplasm, Plasma membrane