제품 > 항체 > 단일클론항체(mAb)

ABflo® 647 Rabbit anti-Human CD1c mAb (A25342)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Flow cytometry: 1X10^6 K-562 cells (negative control, left) and MOLT-4 cells (right) were surface-stained with ABflo® 647 Rabbit anti-Human CD1c mAb (A25342, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 MOLT-4 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human CD1c mAb (A25342, 5 μl/Test, right).

Overview

Product name	ABflo® 647 Rabbit anti-Human CD1c mAb
Catalog No.	A25342
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC65906-ABflo647

Background

This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene is broadly distributed throughout the endocytic system via a tyrosine-based motif in the cytoplasmic tail. Alternatively spliced transcript variants of this gene have been observed, but their full-length nature is not known.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 19-298 of human CD1c (NP_001756.2).
Sequence	ADASQEHVSFHVIQIFSFVNQSWARGQGSGWLDELQTHGWDSESGTIIFLHNWSKGNFSNEELSDLELLFRFYLFGLTREIQDHASQDYSKYPFEVQVKAGCELHSGKSPEGFFQVAFNGLDLLSFQNTTWVPSPGCGSLAQSVCHLLNHQYEGVTETVYNLIRSTCPRFLLGLLDAGKMYVHRQVRPEAWLSSRPSLGSGQLLLVCHASGFYPKPVWVTWMRNEQEQLGTKHGDILPNADGTWYLQVILEVASEEPAGLSCRVRHSSLGGQDIILYWGH
Gene ID
Swiss Prot
Synonyms	R7; CD1; CD1A; BDCA1
Calculated MW	38kDa
Observed MW

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Cell membrane, Endosome membrane, Single-pass type I membrane protein, endoplasmic reticulum, external side of plasma membrane, extracellular space, Golgi apparatus, lysosome, plasma membrane

Documents

Certificate of Compliance

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Lot number

Research Area

연구면역학 & 염증CDs

ABflo® 647 Rabbit anti-Human CD1c mAb images

Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD1c mAb (A25342)

Flow cytometry: 1X10^6 MOLT-4 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human CD1c mAb (A25342, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.