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ABflo® 647 Rabbit anti-Human CD355/CRTAM mAb (A24819)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD355/CRTAM mAb (A24819)

Flow cytometry: 1X10^6 293T cells (negative control, left) and 293T (Transfection, right) cells were surface-stained with ABflo® 647 Rabbit anti-Human CD355/CRTAM mAb (A24819, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD355/CRTAM mAb (A24819)

Flow cytometry: 1X10^6 293T(Transfection) cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human CD355/CRTAM mAb(A24819, 5 μl/Test, right).

Overview

Product nameABflo® 647 Rabbit anti-Human CD355/CRTAM mAb
Catalog No.A24819
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC63867-ABflo647
The CRTAM gene is upregulated in CD4 (see MIM 186940)-positive and CD8 (see CD8A; MIM 186910)-positive T cells and encodes a type I transmembrane protein with V and C1-like Ig domains (Yeh et al., 2008 [PubMed 18329370]).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 18-287 of human CD355/CRTAM (NP_062550.2).
SequenceSLTNHTETITVEEGQTLTLKCVTSLRKNSSLQWLTPSGFTIFLNEYPALKNSKYQLLHHSANQLSITVPNVTLQDEGVYKCLHYSDSVSTKEVKVIVLATPFKPILEASVIRKQNGEEHVVLMCSTMRSKPPPQITWLLGNSMEVSGGTLHEFETDGKKCNTTSTLIIHTYGKNSTVDCIIRHRGLQGRKLVAPFRFEDLVTDEETASDALERNSLSSQDPQQPTSTVSVTEDSSTSEIDKEEKEQTTQDPDLTTEANPQYLGLARKKSG
Gene ID
Swiss Prot
SynonymsCD355
Calculated MW22kDa/44kDa
Observed MWRefer to figures
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationCell membrane; Single-pass type I membrane protein
    ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD355/CRTAM mAb (A24819)}

    Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD355/CRTAM mAb (A24819)

    Flow cytometry: 1X10^6 293T cells (negative control, left) and 293T (Transfection, right) cells were surface-stained with ABflo® 647 Rabbit anti-Human CD355/CRTAM mAb (A24819, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
    ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD355/CRTAM mAb (A24819)}

    Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD355/CRTAM mAb (A24819)

    Flow cytometry: 1X10^6 293T(Transfection) cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human CD355/CRTAM mAb(A24819, 5 μl/Test, right).

    * For research use only. Not for therapeutic or diagnostic purposes.

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