제품 > 항체 > 단일클론항체(mAb)

ABflo® 647 Rabbit anti-Human CD46 mAb (A25551)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Flow cytometry: 1X10^6 knockdown (KD) Hela cells (Low Expression, left) and Hela cells (right) were surface-stained with ABflo® 647 Rabbit anti-Human CD46 mAb (A25551, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 Hela cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human CD46 mAb (A25551, 5 μl/Test, right).

Overview

Product name	ABflo® 647 Rabbit anti-Human CD46 mAb
Catalog No.	A25551
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC65046-ABflo647

Background

The protein encoded by this gene is a type I membrane protein and is a regulatory part of the complement system. The encoded protein has cofactor activity for inactivation of complement components C3b and C4b by serum factor I, which protects the host cell from damage by complement. In addition, the encoded protein can act as a receptor for the Edmonston strain of measles virus, human herpesvirus-6, and type IV pili of pathogenic Neisseria. Finally, the protein encoded by this gene may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations at this locus have been associated with susceptibility to hemolytic uremic syndrome. Alternatively spliced transcript variants encoding different isoforms have been described.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 35-328 of human CD46 (NP_002380.3).
Sequence	CEEPPTFEAMELIGKPKPYYEIGERVDYKCKKGYFYIPPLATHTICDRNHTWLPVSDDACYRETCPYIRDPLNGQAVPANGTYEFGYQMHFICNEGYYLIGEEILYCELKGSVAIWSGKPPICEKVLCTPPPKIKNGKHTFSEVEVFEYLDAVTYSCDPAPGPDPFSLIGESTIYCGDNSVWSRAAPECKVVKCRFPVVENGKQISGFGKKFYYKATVMFECDKGFYLDGSDTIVCDSNSTWDPPVPKCLKVLPPSSTKPPALSHSVSTSSTTKSPASSASGPRPTYKPPVSNY
Gene ID
Swiss Prot
Synonyms	MCP; TLX; AHUS2; MIC10; TRA2.10
Calculated MW	37-44kDa
Observed MW

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Cytoplasmic vesicle, single-pass type I membrane protein, acrosome inner membrane, secretory vesicle

Documents

Certificate of Compliance

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Lot number

Research Area

ABflo® 647 Rabbit anti-Human CD46 mAb images

Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD46 mAb (A25551)

Flow cytometry: 1X10^6 Hela cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human CD46 mAb (A25551, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.