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[KD Validated] CD46 Rabbit mAb (A25553)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Western blot analysis of lysates from wild type (WT) and CD46 knockdown (KD) HeLa cells using [KD Validated] CD46 Rabbit mAb (A25553) at 1:1000 dilution incubated overnight at 4°C.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - [KD Validated] CD46 Rabbit mAb (A25553)

Confocal imaging of K-562 cells using [KD Validated] CD46 Rabbit mAb (A25553, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

Confocal analysis of T-47D cells using [KD Validated] CD46 Rabbit mAb (A25553, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Flow CytoMetry - [KD Validated] CD46 Rabbit mAb (A25553)

Flow cytometry: 1X10^6 knockdown (KD) HeLa cells (Low Expression, left) and HeLa cells (right) were surface-stained with [KD Validated] CD46 Rabbit mAb (A25553, 2 μg/mL, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line), followed by Alexa Fluor® 647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 HeLa cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or [KD Validated] CD46 Rabbit mAb (A25553, 2 μg/mL, right).

Overview

Product name	[KD Validated] CD46 Rabbit mAb
Catalog No.	A25553
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC65046

Background

The protein encoded by this gene is a type I membrane protein and is a regulatory part of the complement system. The encoded protein has cofactor activity for inactivation of complement components C3b and C4b by serum factor I, which protects the host cell from damage by complement. In addition, the encoded protein can act as a receptor for the Edmonston strain of measles virus, human herpesvirus-6, and type IV pili of pathogenic Neisseria. Finally, the protein encoded by this gene may be involved in the fusion of the spermatozoa with the oocyte during fertilization. Mutations at this locus have been associated with susceptibility to hemolytic uremic syndrome. Alternatively spliced transcript variants encoding different isoforms have been described.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 35-328 of human CD46 (NP_002380.3).
Sequence	CEEPPTFEAMELIGKPKPYYEIGERVDYKCKKGYFYIPPLATHTICDRNHTWLPVSDDACYRETCPYIRDPLNGQAVPANGTYEFGYQMHFICNEGYYLIGEEILYCELKGSVAIWSGKPPICEKVLCTPPPKIKNGKHTFSEVEVFEYLDAVTYSCDPAPGPDPFSLIGESTIYCGDNSVWSRAAPECKVVKCRFPVVENGKQISGFGKKFYYKATVMFECDKGFYLDGSDTIVCDSNSTWDPPVPKCLKVLPPSSTKPPALSHSVSTSSTTKSPASSASGPRPTYKPPVSNY
Gene ID
Swiss Prot
Synonyms	MCP; TLX; AHUS2; MIC10; TRA2.10
Calculated MW	37-44kDa
Observed MW	50-70kDa

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:2000 IF/ICC 1:200 - 1:800 FC 1:500 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence Flow Cytometry
Positive samples	HeLa
Cellular location	Cytoplasmic vesicle, single-pass type I membrane protein, acrosome inner membrane, secretory vesicle.

Documents

Certificate of Compliance

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Lot number

Research Area

[KD Validated] CD46 Rabbit mAb images

Western blot - [KD Validated] CD46 Rabbit mAb (A25553)

Immunofluorescence - [KD Validated] CD46 Rabbit mAb (A25553)

Flow CytoMetry - [KD Validated] CD46 Rabbit mAb (A25553)

Flow cytometry: 1X10^6 HeLa cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or [KD Validated] CD46 Rabbit mAb (A25553, 2 μg/mL, right).

* For research use only. Not for therapeutic or diagnostic purposes.