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ACAA2 Rabbit mAb (A25921)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ACAA2 Rabbit mAb (A25921)

Western blot analysis of various lysates using ACAA2 Rabbit mAb (A25921) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - ACAA2 Rabbit mAb (A25921)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using ACAA2 Rabbit mAb (A25921) at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ACAA2 Rabbit mAb (A25921)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using ACAA2 Rabbit mAb (A25921) at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ACAA2 Rabbit mAb (A25921)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using ACAA2 Rabbit mAb (A25921) at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ACAA2 Rabbit mAb (A25921)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using ACAA2 Rabbit mAb (A25921) at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product nameACAA2 Rabbit mAb
Catalog No.A25921
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC68101
The encoded protein catalyzes the last step of the mitochondrial fatty acid beta-oxidation spiral. Unlike most mitochondrial matrix proteins, it contains a non-cleavable amino-terminal targeting signal.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 17-271 of human ACAA2 (NP_006102.2).
SequenceFGAYGGLLKDFTATDLSEFAAKAALSAGKVSPETVDSVIMGNVLQSSSDAIYLARHVGLRVGIPKETPALTINRLCGSGFQSIVNGCQEICVKEAEVVLCGGTESMSQAPYCVRNVRFGTKLGSDIKLEDSLWVSLTDQHVQLPMAMTAENLAVKHKISREECDKYALQSQQRWKAANDAGYFNDEMAPIEVKTKKGKQTMQVDEHARPQTTLEQLQKLPPVFKKDGTVTAGNASGVADGAGAVIIASEDAVKKH
Gene ID
Swiss Prot
SynonymsDSAEC
Calculated MW42kDa
Observed MW42kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:4000
  • IHC-P 1:200 - 1:800
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHep G2, HeLa, 293T, NIH/3T3
Cellular locationMitochondrion.

Documents

Certificate of Compliance

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ABclonal:Western blot - ACAA2 Rabbit mAb (A25921)}

Western blot - ACAA2 Rabbit mAb (A25921)

Western blot analysis of various lysates using ACAA2 Rabbit mAb (A25921) at 1:1000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - ACAA2 Rabbit mAb (A25921)}

Immunohistochemistry - ACAA2 Rabbit mAb (A25921)

Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using ACAA2 Rabbit mAb (A25921) at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ACAA2 Rabbit mAb (A25921)}

Immunohistochemistry - ACAA2 Rabbit mAb (A25921)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using ACAA2 Rabbit mAb (A25921) at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ACAA2 Rabbit mAb (A25921)}

Immunohistochemistry - ACAA2 Rabbit mAb (A25921)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using ACAA2 Rabbit mAb (A25921) at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ACAA2 Rabbit mAb (A25921)}

Immunohistochemistry - ACAA2 Rabbit mAb (A25921)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using ACAA2 Rabbit mAb (A25921) at a dilution of 1:300 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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