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ACAA2 Rabbit mAb (A0664)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ACAA2 Rabbit mAb (A0664)

Western blot analysis of extracts of various cell lines, using ACAA2 antibody (A0664) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - ACAA2 Rabbit mAb (A0664)

Immunofluorescence analysis of HepG2 cells using ACAA2 Rabbit mAb (A0664) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ACAA2 Rabbit mAb (A0664)

Immunofluorescence analysis of NIH/3T3 cells using ACAA2 Rabbit mAb (A0664) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ACAA2 Rabbit mAb (A0664)

Immunofluorescence analysis of U2OS cells using ACAA2 Rabbit mAb (A0664) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameACAA2 Rabbit mAb
Catalog No.A0664
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2533
The encoded protein catalyzes the last step of the mitochondrial fatty acid beta-oxidation spiral. Unlike most mitochondrial matrix proteins, it contains a non-cleavable amino-terminal targeting signal.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 298-397 of human ACAA2 (P42765).
SequencePAISGALKKAGLSLKDMDLVEVNEAFAPQYLAVERSLDLDISKTNVNGGAIALGHPLGGSGSRITAHLVHELRRRGGKYAVGSACIGGGQGIAVIIQSTA
Gene ID
Swiss Prot
SynonymsDSAEC
Calculated MW42kDa
Observed MW42kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHeLa, HepG2, A-549, Rat heart
Cellular locationMitochondrion

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ABclonal:Western blot - ACAA2 Rabbit mAb (A0664)}

Western blot - ACAA2 Rabbit mAb (A0664)

Western blot analysis of extracts of various cell lines, using ACAA2 antibody (A0664) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - ACAA2 Rabbit mAb (A0664)}

Immunofluorescence - ACAA2 Rabbit mAb (A0664)

Immunofluorescence analysis of HepG2 cells using ACAA2 Rabbit mAb (A0664) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ACAA2 Rabbit mAb (A0664)}

Immunofluorescence - ACAA2 Rabbit mAb (A0664)

Immunofluorescence analysis of NIH/3T3 cells using ACAA2 Rabbit mAb (A0664) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ACAA2 Rabbit mAb (A0664)}

Immunofluorescence - ACAA2 Rabbit mAb (A0664)

Immunofluorescence analysis of U2OS cells using ACAA2 Rabbit mAb (A0664) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)