제품 > 항체 > 다중클론항체(pAb)

ACAP2 Rabbit pAb (A12127)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - ACAP2 Rabbit pAb (A12127)

Western blot analysis of various lysates using ACAP2 Rabbit pAb (A12127) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunofluorescence - ACAP2 Rabbit pAb (A12127)

Immunofluorescence analysis of U2OS cells using ACAP2 Rabbit pAb (A12127) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameACAP2 Rabbit pAb
Catalog No.A12127
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables GTPase activator activity. Acts upstream of or within actin filament-based process. Located in ruffle.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 50-160 of human ACAP2 (NP_036419.3).
SequenceKAFCVANKQFMNGIRDLAQYSSNDAVVETSLTKFSDSLQEMINFHTILFDQTQRSIKAQLQNFVKEDLRKFKDAKKQFEKVSEEKENALVKNAQVQRNKQHEVEEATNILT
Gene ID
Swiss Prot
SynonymsCENTB2; CNT-B2; ACAP2
Calculated MW88kDa
Observed MW115kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesLO2, HeLa, Jurkat, U-87MG, A-549, Mouse brain, Mouse liver
Cellular locationEndosome membrane, Peripheral membrane protein

Documents

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    ABclonal:Western blot - ACAP2 Rabbit pAb (A12127)}

    Western blot - ACAP2 Rabbit pAb (A12127)

    Western blot analysis of various lysates using ACAP2 Rabbit pAb (A12127) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 5s.
    ABclonal:Immunofluorescence - ACAP2 Rabbit pAb (A12127)}

    Immunofluorescence - ACAP2 Rabbit pAb (A12127)

    Immunofluorescence analysis of U2OS cells using ACAP2 Rabbit pAb (A12127) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.