제품 > 항체 > 다중클론항체(pAb)

ACOT9 Rabbit pAb (A15416)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ACOT9 Rabbit pAb (A15416)

Western blot analysis of various lysates using ACOT9 Rabbit pAb (A15416) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - ACOT9 Rabbit pAb (A15416)

Immunofluorescence analysis of A431 cells using ACOT9 Rabbit pAb (A15416) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameACOT9 Rabbit pAb
Catalog No.A15416
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a mitochondrial acyl-CoA thioesterase of unknown function. Two transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 22-250 of human ACOT9 (NP_001028755.2).
SequenceLTQGPQNPKKQGIFHIHEVRDKLREIVGASTNWRDHVKAMEERKLLHSFLAKSQDGLPPRRMKDSYIEVLLPLGSEPELREKYLTVQNTVRFGRILEDLDSLGVLICYMHNKIHSAKMSPLSIVTALVDKIDMCKKSLSPEQDIKFSGHVSWVGKTSMEVKMQMFQLHGDEFCPVLDATFVMVARDSENKGPAFVNPLIPESPEEEELFRQGELNKGRRIAFSSTSLLK
Gene ID
Swiss Prot
SynonymsACATE2; CGI-16; MTACT48; MT-ACT48; ACOT9
Calculated MW50kDa
Observed MW50kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:200 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesU-251MG, MCF7, HT-29, Mouse brain, Mouse heart, Mouse kidney, Mouse lung, Rat pancreas
Cellular locationMitochondrion

Documents

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ABclonal:Western blot - ACOT9 Rabbit pAb (A15416)}

Western blot - ACOT9 Rabbit pAb (A15416)

Western blot analysis of various lysates using ACOT9 Rabbit pAb (A15416) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - ACOT9 Rabbit pAb (A15416)}

Immunofluorescence - ACOT9 Rabbit pAb (A15416)

Immunofluorescence analysis of A431 cells using ACOT9 Rabbit pAb (A15416) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.