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ACTN1 Rabbit pAb (A1160)

Publications (8) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse

ABclonal:Western blot - ACTN1 Rabbit pAb (A1160)

Western blot analysis of lysates from NIH/3T3 cells, using ACTN1 Rabbit pAb (A1160) at 1:900 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - ACTN1 Rabbit pAb (A1160)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using ACTN1 Rabbit pAb (A1160) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ACTN1 Rabbit pAb (A1160)

Immunohistochemistry analysis of paraffin-embedded human esophagus using ACTN1 Rabbit pAb (A1160) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ACTN1 Rabbit pAb (A1160)

Immunohistochemistry analysis of paraffin-embedded mouse heart using ACTN1 Rabbit pAb (A1160) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameACTN1 Rabbit pAb
Catalog No.A1160
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-330 of human ACTN1 (NP_001123477.1).
SequenceMDHYDSQQTNDYMQPEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAEKYLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEQLMEDYEKLASDLLEWIRRTIPWLENRVPENTMHAMQQKLEDFRDYRRLHKPPKVQE
Gene ID
Swiss Prot
SynonymsBDPLT15; ACTN1
Calculated MW103kDa
Observed MW103kDa
ReactivityHuman, Mouse
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesNIH/3T3
Cellular locationCell junction, Cell membrane, Cell projection, Cytoplasm, Z line, cytoskeleton, myofibril, ruffle, sarcomere
Customer validation

IF(Homo sapiens)

WB(Mus musculus, Danio rerio, Gallus gallus, Homo sapiens)

IF(Gallus gallus)

Documents

Certificate of Compliance

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ABclonal:Western blot - ACTN1 Rabbit pAb (A1160)}

Western blot - ACTN1 Rabbit pAb (A1160)

Western blot analysis of lysates from NIH/3T3 cells, using ACTN1 Rabbit pAb (A1160) at 1:900 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - ACTN1 Rabbit pAb (A1160)}

Immunohistochemistry - ACTN1 Rabbit pAb (A1160)

Immunohistochemistry analysis of paraffin-embedded human liver cancer using ACTN1 Rabbit pAb (A1160) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - ACTN1 Rabbit pAb (A1160)}

Immunohistochemistry - ACTN1 Rabbit pAb (A1160)

Immunohistochemistry analysis of paraffin-embedded human esophagus using ACTN1 Rabbit pAb (A1160) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - ACTN1 Rabbit pAb (A1160)}

Immunohistochemistry - ACTN1 Rabbit pAb (A1160)

Immunohistochemistry analysis of paraffin-embedded mouse heart using ACTN1 Rabbit pAb (A1160) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A1160? Please let us know so that we can cite the reference in this datasheet.

ELISA 키트 (1)

항체 (1)

Secondary Antibodies (26)