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ACVR2A Rabbit pAb (A1981)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ACVR2A Rabbit pAb (A1981)

Western blot analysis of lysates from A-431 cells, using ACVR2A Rabbit pAb (A1981) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

ABclonal:Immunohistochemistry - ACVR2A Rabbit pAb (A1981)

Immunohistochemistry analysis of paraffin-embedded Human lung using ACVR2A Rabbit pAb (A1981) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - ACVR2A Rabbit pAb (A1981)

Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using ACVR2A Rabbit pAb (A1981) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunohistochemistry - ACVR2A Rabbit pAb (A1981)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using ACVR2A Rabbit pAb (A1981) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - ACVR2A Rabbit pAb (A1981)

Immunofluorescence analysis of NIH/3T3 cells using ACVR2A Rabbit pAb (A1981) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameACVR2A Rabbit pAb
Catalog No.A1981
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a receptor that mediates the functions of activins, which are members of the transforming growth factor-beta (TGF-beta) superfamily involved in diverse biological processes. The encoded protein is a transmembrane serine-threonine kinase receptor which mediates signaling by forming heterodimeric complexes with various combinations of type I and type II receptors and ligands in a cell-specific manner. The encoded type II receptor is primarily involved in ligand-binding and includes an extracellular ligand-binding domain, a transmembrane domain and a cytoplasmic serine-threonine kinase domain. This gene may be associated with susceptibility to preeclampsia, a pregnancy-related disease which can result in maternal and fetal morbidity and mortality. Alternative splicing results in multiple transcript variants of this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 20-140 of human ACVR2A (NP_001607.1).
SequenceAILGRSETQECLFFNANWEKDRTNQTGVEPCYGDKDKRRHCFATWKNISGSIEIVKQGCWLDDINCYDRTDCVEKKDSPEVYFCCCEGNMCNEKFSYFPEMEVTQPTSNPVTPKPPYYNIL
Gene ID
Swiss Prot
SynonymsACVR2; ACTRII; ACVR2A
Calculated MW58kDa
Observed MW58kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IHC-P 1:100 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesA-431
Cellular locationMembrane, Single-pass type I membrane protein.
Customer validation

IF(Homo sapiens)

WB(Homo sapiens)

WB(Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - ACVR2A Rabbit pAb (A1981)}

Western blot - ACVR2A Rabbit pAb (A1981)

Western blot analysis of lysates from A-431 cells, using ACVR2A Rabbit pAb (A1981) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.
ABclonal:Immunohistochemistry - ACVR2A Rabbit pAb (A1981)}

Immunohistochemistry - ACVR2A Rabbit pAb (A1981)

Immunohistochemistry analysis of paraffin-embedded Human lung using ACVR2A Rabbit pAb (A1981) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - ACVR2A Rabbit pAb (A1981)}

Immunohistochemistry - ACVR2A Rabbit pAb (A1981)

Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using ACVR2A Rabbit pAb (A1981) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunohistochemistry - ACVR2A Rabbit pAb (A1981)}

Immunohistochemistry - ACVR2A Rabbit pAb (A1981)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using ACVR2A Rabbit pAb (A1981) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
ABclonal:Immunofluorescence - ACVR2A Rabbit pAb (A1981)}

Immunofluorescence - ACVR2A Rabbit pAb (A1981)

Immunofluorescence analysis of NIH/3T3 cells using ACVR2A Rabbit pAb (A1981) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A1981? Please let us know so that we can cite the reference in this datasheet.

항체 (1)

재조합 단백질 (1)

Secondary Antibodies (26)