제품 > 항체 > 다중클론항체(pAb)

ADAP1 Rabbit pAb (A4479)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ADAP1 Rabbit pAb (A4479)

Western blot analysis of extracts of various cell lines, using ADAP1 antibody (A4479) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - ADAP1 Rabbit pAb (A4479)

Immunofluorescence analysis of U2OS cells using ADAP1 antibody (A4479) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameADAP1 Rabbit pAb
Catalog No.A4479
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables GTPase activator activity. Involved in regulation of GTPase activity. Located in cytosol; nucleus; and plasma membrane.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-374 of human ADAP1 (NP_006860.1).
SequenceMAKERRRAVLELLQRPGNARCADCGAPDPDWASYTLGVFICLSCSGIHRNIPQVSKVKSVRLDAWEEAQVEFMASHGNDAARARFESKVPSFYYRPTPSDCQLLREQWIRAKYERQEFIYPEKQEPYSAGYREGFLWKRGRDNGQFLSRKFVLTEREGALKYFNRNDAKEPKAVMKIEHLNATFQPAKIGHPHGLQVTYLKDNSTRNIFIYHEDGKEIVDWFNALRAARFHYLQVAFPGASDADLVPKLSRNYLKEGYMEKTGPKQTEGFRKRWFTMDDRRLMYFKDPLDAFARGEVFIGSKESGYTVLHGFPPSTQGHHWPHGITIVTPDRKFLFACETESDQREWVAAFQKAVDRPMLPQEYAVEAHFKHKP
Gene ID
Swiss Prot
SynonymsGCS1L; CENTA1; p42IP4
Calculated MW43kDa
Observed MW43kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesBT-474, Jurkat, Mouse small intestine, Rat kidney
Cellular locationCytoplasm, Nucleus

Documents

Certificate of Compliance

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    ABclonal:Western blot - ADAP1 Rabbit pAb (A4479)}

    Western blot - ADAP1 Rabbit pAb (A4479)

    Western blot analysis of extracts of various cell lines, using ADAP1 antibody (A4479) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.
    ABclonal:Immunofluorescence - ADAP1 Rabbit pAb (A4479)}

    Immunofluorescence - ADAP1 Rabbit pAb (A4479)

    Immunofluorescence analysis of U2OS cells using ADAP1 antibody (A4479) at dilution of 1:100. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)