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ADH5/GSNOR Rabbit pAb (A13459)

Publications (2) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ADH5/GSNOR Rabbit pAb (A13459)

Western blot analysis of various lysates using ADH5/GSNOR Rabbit pAb (A13459) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - ADH5/GSNOR Rabbit pAb (A13459)

Immunofluorescence analysis of HeLa cells using ADH5/GSNOR Rabbit pAb (A13459) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ADH5/GSNOR Rabbit pAb (A13459)

Immunofluorescence analysis of HepG2 cells using ADH5/GSNOR Rabbit pAb (A13459) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ADH5/GSNOR Rabbit pAb (A13459)

Immunofluorescence analysis of NIH/3T3 cells using ADH5/GSNOR Rabbit pAb (A13459) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ADH5/GSNOR Rabbit pAb (A13459)

Immunofluorescence analysis of PC-12 cells using ADH5/GSNOR Rabbit pAb (A13459) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameADH5/GSNOR Rabbit pAb
Catalog No.A13459
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The encoded protein forms a homodimer. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis. The human genome contains several non-transcribed pseudogenes related to this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-374 of human ADH5/GSNOR (NP_000662.3).
SequenceMANEVIKCKAAVAWEAGKPLSIEEIEVAPPKAHEVRIKIIATAVCHTDAYTLSGADPEGCFPVILGHEGAGIVESVGEGVTKLKAGDTVIPLYIPQCGECKFCLNPKTNLCQKIRVTQGKGLMPDGTSRFTCKGKTILHYMGTSTFSEYTVVADISVAKIDPLAPLDKVCLLGCGISTGYGAAVNTAKLEPGSVCAVFGLGGVGLAVIMGCKVAGASRIIGVDINKDKFARAKEFGATECINPQDFSKPIQEVLIEMTDGGVDYSFECIGNVKVMRAALEACHKGWGVSVVVGVAASGEEIATRPFQLVTGRTWKGTAFGGWKSVESVPKLVSEYMSKKIKVDEFVTHNLSFDEINKAFELMHSGKSIRTVVKI
Gene ID
Swiss Prot
SynonymsFDH; ADHX; ADH-3; AMEDS; BMFS7; FALDH; GSNOR; GSH-FDH; HEL-S-60p; ADH5/GSNOR
Calculated MW40kDa
Observed MW40kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesK-562, Mouse brain, Rat liver, Rat kidney
Cellular locationCytoplasm.
Customer validation

WB(Mus musculus, Homo sapiens)

Documents

Certificate of Compliance

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ABclonal:Western blot - ADH5/GSNOR Rabbit pAb (A13459)}

Western blot - ADH5/GSNOR Rabbit pAb (A13459)

Western blot analysis of various lysates using ADH5/GSNOR Rabbit pAb (A13459) at 1:500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunofluorescence - ADH5/GSNOR Rabbit pAb (A13459)}

Immunofluorescence - ADH5/GSNOR Rabbit pAb (A13459)

Immunofluorescence analysis of HeLa cells using ADH5/GSNOR Rabbit pAb (A13459) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ADH5/GSNOR Rabbit pAb (A13459)}

Immunofluorescence - ADH5/GSNOR Rabbit pAb (A13459)

Immunofluorescence analysis of HepG2 cells using ADH5/GSNOR Rabbit pAb (A13459) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ADH5/GSNOR Rabbit pAb (A13459)}

Immunofluorescence - ADH5/GSNOR Rabbit pAb (A13459)

Immunofluorescence analysis of NIH/3T3 cells using ADH5/GSNOR Rabbit pAb (A13459) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ADH5/GSNOR Rabbit pAb (A13459)}

Immunofluorescence - ADH5/GSNOR Rabbit pAb (A13459)

Immunofluorescence analysis of PC-12 cells using ADH5/GSNOR Rabbit pAb (A13459) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A13459? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

Secondary Antibodies (26)