제품 > 항체 > 다중클론항체(pAb)

ADH5/GSNOR Rabbit pAb (A2041)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using ADH5/GSNOR Rabbit pAb (A2041) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

ABclonal:Immunofluorescence - ADH5/GSNOR Rabbit pAb (A2041)

Immunofluorescence analysis of SH-SY5Y cells using ADH5/GSNOR Rabbit pAb(A2041) at a dilution of 1:50 (40x lens). Secondary antibody:() at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using ADH5/GSNOR Rabbit pAb(A2041) at a dilution of 1:50 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of NIH/3T3 cells using ADH5/GSNOR Rabbit pAb(A2041) at a dilution of 1:50 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of Hep G2 cells using ADH5/GSNOR Rabbit pAb(A2041) at a dilution of 1:50 (40x lens). Secondary antibody:Cy3 Goat Anti-Rabbit IgG (H+L)(AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	ADH5/GSNOR Rabbit pAb
Catalog No.	A2041
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The encoded protein forms a homodimer. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis. The human genome contains several non-transcribed pseudogenes related to this gene.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-374 of human ADH5/GSNOR (NP_000662.3).
Sequence	MANEVIKCKAAVAWEAGKPLSIEEIEVAPPKAHEVRIKIIATAVCHTDAYTLSGADPEGCFPVILGHEGAGIVESVGEGVTKLKAGDTVIPLYIPQCGECKFCLNPKTNLCQKIRVTQGKGLMPDGTSRFTCKGKTILHYMGTSTFSEYTVVADISVAKIDPLAPLDKVCLLGCGISTGYGAAVNTAKLEPGSVCAVFGLGGVGLAVIMGCKVAGASRIIGVDINKDKFARAKEFGATECINPQDFSKPIQEVLIEMTDGGVDYSFECIGNVKVMRAALEACHKGWGVSVVVGVAASGEEIATRPFQLVTGRTWKGTAFGGWKSVESVPKLVSEYMSKKIKVDEFVTHNLSFDEINKAFELMHSGKSIRTVVKI
Gene ID
Swiss Prot
Synonyms	FDH; ADHX; ADH-3; AMEDS; BMFS7; FALDH; GSNOR; GSH-FDH; HEL-S-60p; ADH5/GSNOR
Calculated MW	40kDa
Observed MW

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	U-251MG, U-937
Cellular location	Cytoplasm
Customer validation	IF(Mus musculus)

Documents

Certificate of Compliance

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Research Area

연구면역학 & 염증CDs

ADH5/GSNOR Rabbit pAb images

Western blot - ADH5/GSNOR Rabbit pAb (A2041)

Immunofluorescence - ADH5/GSNOR Rabbit pAb (A2041)

Immunofluorescence analysis of SH-SY5Y cells using ADH5/GSNOR Rabbit pAb(A2041) at a dilution of 1:50 (40x lens). Secondary antibody:() at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence - ADH5/GSNOR Rabbit pAb (A2041)

* For research use only. Not for therapeutic or diagnostic purposes.