제품 > 항체 > 다중클론항체(pAb)

AFG3L2 Rabbit pAb (A23598)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates, using AFG3L2 Rabbit pAb (A23598) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - AFG3L2 Rabbit pAb (A23598)

Immunohistochemistry analysis of AFG3L2 in paraffin-embedded Human colon carcinoma tissue using AFG3L2 Rabbit pAb (A23598) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunoprecipitation - AFG3L2 Rabbit pAb (A23598)

Immunoprecipitation analysis of 600ug extracts of Mouse liver using 3ug AFG3L2 Rabbit pAb (A23598 1:100). Western blot was performed from the immunoprecipitate using AFG3L2 Rabbit pAb (A23598) at a dilition of 1:500.

Overview

Product name	AFG3L2 Rabbit pAb
Catalog No.	A23598
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a protein localized in mitochondria and closely related to paraplegin. The paraplegin gene is responsible for an autosomal recessive form of hereditary spastic paraplegia. This gene is a candidate gene for other hereditary spastic paraplegias or neurodegenerative disorders.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 538-797 of human AFG3L2（NP_006787.2）.
Sequence	DSINQKHFEQAIERVIGGLEKKTQVLQPEEKKTVAYHEAGHAVAGWYLEHADPLLKVSIIPRGKGLGYAQYLPKEQYLYTKEQLLDRMCMTLGGRVSEEIFFGRITTGAQDDLRKVTQSAYAQIVQFGMNEKVGQISFDLPRQGDMVLEKPYSEATARLIDDEVRILINDAYKRTVALLTEKKADVEKVALLLLEKEVLDKNDMVELLGPRPFAEKSTYEEFVEGTGSLDEDTSLPEGLKDWNKEREKEKEEPPGEKVAN
Gene ID
Swiss Prot
Synonyms	OPA12; SCA28; SPAX5; AFG3L2
Calculated MW	89kDa
Observed MW	80kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IP 0.5μg-4μg antibody for 400μg-600μg extracts of whole cells
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunoprecipitation
Positive samples	Jurkat, 293T, Mouse liver
Cellular location	Mitochondrion membrane, Multi-pass membrane protein