제품 > 항체 > 단일클론항체(mAb)

AGER Rabbit mAb (A23423)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of various lysates, using AGER Rabbit mAb (A23423) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - AGER Rabbit mAb (A23423)

Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.Immunofluorescence analysis of human lung cells using AGER Rabbit mAb (A23423) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product name	AGER Rabbit mAb
Catalog No.	A23423
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC61005

Background

The advanced glycosylation end product (AGE) receptor encoded by this gene is a member of the immunoglobulin superfamily of cell surface receptors. It is a multiligand receptor, and besides AGE, interacts with other molecules implicated in homeostasis, development, and inflammation, and certain diseases, such as diabetes and Alzheimer's disease. Many alternatively spliced transcript variants encoding different isoforms, as well as non-protein-coding variants, have been described for this gene (PMID:18089847).

Immunogen information

Immunogen	Recombinant protein of human AGER.
Sequence	Email for sequence
Gene ID
Swiss Prot
Synonyms	RAGE; sRAGE; SCARJ1
Calculated MW	43kDa
Observed MW	52kDa/48kDa/58kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:5000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	OVCAR3, K-562(Negative control)
Cellular location	Cell membrane, Secreted, Single-pass type I membrane protein