제품 > 항체 > 다중클론항체(pAb)

AGTRAP Rabbit pAb (A12178)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - AGTRAP Rabbit pAb (A12178)

Western blot analysis of various lysates using AGTRAP Rabbit pAb (A12178) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - AGTRAP Rabbit pAb (A12178)

Immunofluorescence analysis of U2OS cells using AGTRAP Rabbit pAb (A12178) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameAGTRAP Rabbit pAb
Catalog No.A12178
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a transmembrane protein localized to the plasma membrane and perinuclear vesicular structures. The gene product interacts with the angiotensin II type I receptor and negatively regulates angiotensin II signaling. Alternative splicing of this gene generates multiple transcript variants encoding different isoforms.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 93-152 of human AGTRAP (NP_065083.3).
SequenceILSLLLKPLSCCFVYHMYRERGGELLVHTGFLGSSQDRSAYQTIDSAEAPADPFAVPEGR
Gene ID
Swiss Prot
SynonymsATRAP; AGTRAP
Calculated MW17kDa
Observed MW20kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samples293T, HT-29, U-87MG, BxPC-3, Mouse kidney, Mouse testis, Mouse heart, Rat kidney, Rat heart
Cellular locationCytoplasmic vesicle membrane, Endoplasmic reticulum membrane, Golgi apparatus membrane, Multi-pass membrane protein

Documents

Certificate of Compliance

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ABclonal:Western blot - AGTRAP Rabbit pAb (A12178)}

Western blot - AGTRAP Rabbit pAb (A12178)

Western blot analysis of various lysates using AGTRAP Rabbit pAb (A12178) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - AGTRAP Rabbit pAb (A12178)}

Immunofluorescence - AGTRAP Rabbit pAb (A12178)

Immunofluorescence analysis of U2OS cells using AGTRAP Rabbit pAb (A12178) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.