제품 > 항체 > 다중클론항체(pAb)

AIMP1 Rabbit pAb (A5301)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - AIMP1 Rabbit pAb (A5301)

Western blot analysis of various lysates using AIMP1 Rabbit pAb (A5301) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - AIMP1 Rabbit pAb (A5301)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using AIMP1 Rabbit pAb (A5301) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - AIMP1 Rabbit pAb (A5301)

Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using AIMP1 Rabbit pAb (A5301) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - AIMP1 Rabbit pAb (A5301)

Immunohistochemistry analysis of paraffin-embedded Mouse heart using AIMP1 Rabbit pAb (A5301) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameAIMP1 Rabbit pAb
Catalog No.A5301
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a cytokine that is specifically induced by apoptosis, and it is involved in the control of angiogenesis, inflammation, and wound healing. The release of this cytokine renders the tumor-associated vasculature sensitive to tumor necrosis factor. The precursor protein is identical to the p43 subunit, which is associated with the multi-tRNA synthetase complex, and it modulates aminoacylation activity of tRNA synthetase in normal cells. This protein is also involved in the stimulation of inflammatory responses after proteolytic cleavage in tumor cells. Multiple transcript variants encoding different isoforms have been found for this gene. A pseudogene has been identified on chromosome 20.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 25-180 of human AIMP1 (NP_001135888.1).
SequenceMANNDAVLKRLEQKGAEADQIIEYLKQQVSLLKEKAILQATLREEKKLRVENAKLKKEIEELKQELIQAEIQNGVKQIPFPSGTPLHANSMVSENVIQSTAVTTVSSGTKEQIKGGTGDEKKAKEKIEKKGEKKEKKQQSIAGSADSKPIDVSRLD
Gene ID
Swiss Prot
Synonymsp43; HLD3; EMAP2; SCYE1; EMAPII; AIMP1
Calculated MW34kDa
Observed MW37kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHL-60, 22RV1, HepG2, HT-29, MCF7, A-549
Cellular locationCytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum, Golgi apparatus, Nucleus, Secreted, cytosol, secretory vesicle

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - AIMP1 Rabbit pAb (A5301)}

Western blot - AIMP1 Rabbit pAb (A5301)

Western blot analysis of various lysates using AIMP1 Rabbit pAb (A5301) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - AIMP1 Rabbit pAb (A5301)}

Immunohistochemistry - AIMP1 Rabbit pAb (A5301)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using AIMP1 Rabbit pAb (A5301) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - AIMP1 Rabbit pAb (A5301)}

Immunohistochemistry - AIMP1 Rabbit pAb (A5301)

Immunohistochemistry analysis of paraffin-embedded Human gastric cancer using AIMP1 Rabbit pAb (A5301) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - AIMP1 Rabbit pAb (A5301)}

Immunohistochemistry - AIMP1 Rabbit pAb (A5301)

Immunohistochemistry analysis of paraffin-embedded Mouse heart using AIMP1 Rabbit pAb (A5301) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)