제품 > 항체 > 다중클론항체(pAb)

AK5 Rabbit pAb (A15424)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - AK5 Rabbit pAb (A15424)

Western blot analysis of various lysates using AK5 Rabbit pAb (A15424) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - AK5 Rabbit pAb (A15424)

Immunohistochemistry analysis of paraffin-embedded rat brain using AK5 Rabbit pAb (A15424) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameAK5 Rabbit pAb
Catalog No.A15424
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the adenylate kinase family, which is involved in regulating the adenine nucleotide composition within a cell by catalyzing the reversible transfer of phosphate groups among adenine nucleotides. This member is related to the UMP/CMP kinase of several species. It is located in the cytosol and expressed exclusively in brain. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 150-370 of human AK5 (NP_777283.1).
SequenceLKIAERYGFQYISVGELLRKKIHSTSSNRKWSLIAKIITTGELAPQETTITEIKQKLMQIPDEEGIVIDGFPRDVAQALSFEDQICTPDLVVFLACANQRLKERLLKRAEQQGRPDDNVKATQRRLMNFKQNAAPLVKYFQEKGLIMTFDADRDEDEVFYDISMAVDNKLFPNKEAAAGSSDLDPSMILDTGEIIDTGSDYEDQGDDQLNVFGEDTMGGFM
Gene ID
Swiss Prot
SynonymsAK6; AK5
Calculated MW63kDa
Observed MW63kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:200 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesLO2, Jurkat, Mouse liver, Rat liver
Cellular locationCytoplasm

Documents

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    ABclonal:Western blot - AK5 Rabbit pAb (A15424)}

    Western blot - AK5 Rabbit pAb (A15424)

    Western blot analysis of various lysates using AK5 Rabbit pAb (A15424) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunohistochemistry - AK5 Rabbit pAb (A15424)}

    Immunohistochemistry - AK5 Rabbit pAb (A15424)

    Immunohistochemistry analysis of paraffin-embedded rat brain using AK5 Rabbit pAb (A15424) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.