제품 > 항체 > 다중클론항체(pAb)

AKR7A3 Rabbit pAb (A8194)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - AKR7A3 Rabbit pAb (A8194)

Western blot analysis of extracts of various cell lines, using AKR7A3 antibody (A8194) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product nameAKR7A3 Rabbit pAb
Catalog No.A8194
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Aldo-keto reductases, such as AKR7A3, are involved in the detoxification of aldehydes and ketones.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-331 of human AKR7A3 (NP_036199.2).
SequenceMSRQLSRARPATVLGAMEMGRRMDAPTSAAVTRAFLERGHTEIDTAFVYSEGQSETILGGLGLRLGGSDCRVKIDTKAIPLFGNSLKPDSLRFQLETSLKRLQCPRVDLFYLHMPDHSTPVEETLRACHQLHQEGKFVELGLSNYAAWEVAEICTLCKSNGWILPTVYQGMYNAITRQVETELFPCLRHFGLRFYAFNPLAGGLLTGKYKYEDKNGKQPVGRFFGNTWAEMYRNRYWKEHHFEGIALVEKALQAAYGASAPSMTSATLRWMYHHSQLQGAHGDAVILGMSSLEQLEQNLAAAEEGPLEPAVVDAFNQAWHLVTHECPNYFR
Gene ID
Swiss Prot
SynonymsAFAR2
Calculated MW37kDa
Observed MW37kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesHepG2, HT-29, Mouse kidney, Mouse liver, Mouse pancreas
Cellular locationCytoplasm

Documents

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    ABclonal:Western blot - AKR7A3 Rabbit pAb (A8194)}

    Western blot - AKR7A3 Rabbit pAb (A8194)

    Western blot analysis of extracts of various cell lines, using AKR7A3 antibody (A8194) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 30s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)