제품 > 항체 > 다중클론항체(pAb)

AKTIP Rabbit pAb (A6239)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - AKTIP Rabbit pAb (A6239)

Western blot analysis of various lysates using AKTIP Rabbit pAb (A6239) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 40s.

ABclonal:Immunohistochemistry - AKTIP Rabbit pAb (A6239)

Immunohistochemistry analysis of paraffin-embedded Rat brain using AKTIP Rabbit pAb (A6239) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameAKTIP Rabbit pAb
Catalog No.A6239
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The mouse homolog of this gene produces fused toes and thymic hyperplasia in heterozygous mutant animals while homozygous mutants die in early development. This gene may play a role in apoptosis as these morphological abnormalities are caused by altered patterns of programmed cell death. The protein encoded by this gene is similar to the ubiquitin ligase domain of other ubiquitin-conjugating enzymes but lacks the conserved cysteine residue that enables those enzymes to conjugate ubiquitin to the target protein. This protein interacts directly with serine/threonine kinase protein kinase B (PKB)/Akt and modulates PKB activity by enhancing the phosphorylation of PKB's regulatory sites. Alternative splicing results in two transcript variants encoding the same protein.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-292 of human AKTIP (NP_071921.1).
SequenceMNPFWSMSTSSVRKRSEGEEKTLTGDVKTSPPRTAPKKQLPSIPKNALPITKPTSPAPAAQSTNGTHASYGPFYLEYSLLAEFTLVVKQKLPGVYVQPSYRSALMWFGVIFIRHGLYQDGVFKFTVYIPDNYPDGDCPRLVFDIPVFHPLVDPTSGELDVKRAFAKWRRNHNHIWQVLMYARRVFYKIDTASPLNPEAAVLYEKDIQLFKSKVVDSVKVCTARLFDQPKIEDPYAISFSPWNPSVHDEAREKMLTQKKPEEQHNKSVHVAGLSWVKPGSVQPFSKEEKTVAT
Gene ID
Swiss Prot
SynonymsFT1; FTS; AKTIP
Calculated MW33kDa
Observed MW36kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHepG2, MCF7, 293T, Mouse brain, Rat kidney
Cellular locationCell membrane, Cytoplasm, Peripheral membrane protein

Documents

Certificate of Compliance

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    ABclonal:Western blot - AKTIP Rabbit pAb (A6239)}

    Western blot - AKTIP Rabbit pAb (A6239)

    Western blot analysis of various lysates using AKTIP Rabbit pAb (A6239) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 40s.
    ABclonal:Immunohistochemistry - AKTIP Rabbit pAb (A6239)}

    Immunohistochemistry - AKTIP Rabbit pAb (A6239)

    Immunohistochemistry analysis of paraffin-embedded Rat brain using AKTIP Rabbit pAb (A6239) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)