제품 > 항체 > 다중클론항체(pAb)

ALDH2 Rabbit pAb (A1226)

Review (1)Publications (4) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ALDH2 Rabbit pAb (A1226)

Western blot analysis of various lysates using ALDH2 Rabbit pAb (A1226) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - ALDH2 Rabbit pAb (A1226)

Immunohistochemistry analysis of paraffin-embedded human breast cancer using ALDH2 Rabbit pAb (A1226) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ALDH2 Rabbit pAb (A1226)

Immunohistochemistry analysis of paraffin-embedded human liver using ALDH2 Rabbit pAb (A1226) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - ALDH2 Rabbit pAb (A1226)

Immunofluorescence analysis of NIH/3T3 cells using ALDH2 Rabbit pAb (A1226) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ALDH2 Rabbit pAb (A1226)

Immunofluorescence analysis of PC-12 cells using ALDH2 Rabbit pAb (A1226) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ALDH2 Rabbit pAb (A1226)

Immunofluorescence analysis of U2OS cells using ALDH2 Rabbit pAb (A1226) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review
ABclonal: review for ALDH2 Rabbit pAb(A1226)

Overview

Product nameALDH2 Rabbit pAb
Catalog No.A1226
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of aldehyde dehydrogenase, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of East Asians have the cytosolic isozyme but not the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among East Asians than among Caucasians could be related to the absence of a catalytically active form of the mitochondrial isozyme. The increased exposure to acetaldehyde in individuals with the catalytically inactive form may also confer greater susceptibility to many types of cancer. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 321-480 of human ALDH2 (NP_000681.2).
SequenceAGSRTFVQEDIYDEFVERSVARAKSRVVGNPFDSKTEQGPQVDETQFKKILGYINTGKQEGAKLLCGGGIAADRGYFIQPTVFGDVQDGMTIAKEEIFGPVMQILKFKTIEEVVGRANNSTYGLAAAVFTKDLDKANYLSQALQAGTVWVNCYDVFGAQS
Gene ID
Swiss Prot
SynonymsALDM; ALDHI; ALDH-E2; ALDH2
Calculated MW56kDa
Observed MW50-56kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHepG2, A-549, Mouse liver, Mouse kidney, Rat liver, Rat testis
Cellular locationMitochondrion matrix
Customer validation

(Mouse brain)

WB(Homo sapiens, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

ABclonal:Western blot - ALDH2 Rabbit pAb (A1226)}

Western blot - ALDH2 Rabbit pAb (A1226)

Western blot analysis of various lysates using ALDH2 Rabbit pAb (A1226) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - ALDH2 Rabbit pAb (A1226)}

Immunohistochemistry - ALDH2 Rabbit pAb (A1226)

Immunohistochemistry analysis of paraffin-embedded human breast cancer using ALDH2 Rabbit pAb (A1226) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - ALDH2 Rabbit pAb (A1226)}

Immunohistochemistry - ALDH2 Rabbit pAb (A1226)

Immunohistochemistry analysis of paraffin-embedded human liver using ALDH2 Rabbit pAb (A1226) at dilution of 1:200 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - ALDH2 Rabbit pAb (A1226)}

Immunofluorescence - ALDH2 Rabbit pAb (A1226)

Immunofluorescence analysis of NIH/3T3 cells using ALDH2 Rabbit pAb (A1226) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ALDH2 Rabbit pAb (A1226)}

Immunofluorescence - ALDH2 Rabbit pAb (A1226)

Immunofluorescence analysis of PC-12 cells using ALDH2 Rabbit pAb (A1226) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ALDH2 Rabbit pAb (A1226)}

Immunofluorescence - ALDH2 Rabbit pAb (A1226)

Immunofluorescence analysis of U2OS cells using ALDH2 Rabbit pAb (A1226) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A1226

Please submit reviews to your technical sales specialist directly. Alternatively, email us at info@abclonal.co.kr

Reviews List

Publishing research using A1226? Please let us know so that we can cite the reference in this datasheet.

항체 (2)

Secondary Antibodies (26)