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ALDH6A1 Rabbit pAb (A14750)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Immunohistochemistry - ALDH6A1 Rabbit pAb (A14750)

Immunohistochemistry analysis of paraffin-embedded rat ovary using ALDH6A1 Rabbit pAb (A14750) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ALDH6A1 Rabbit pAb (A14750)

Immunohistochemistry analysis of paraffin-embedded human stomach using ALDH6A1 Rabbit pAb (A14750) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ALDH6A1 Rabbit pAb (A14750)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using ALDH6A1 Rabbit pAb (A14750) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - ALDH6A1 Rabbit pAb (A14750)

Immunofluorescence analysis of HeLa cells using ALDH6A1 Rabbit pAb (A14750) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ALDH6A1 Rabbit pAb (A14750)

Immunofluorescence analysis of NIH-3T3 cells using ALDH6A1 Rabbit pAb (A14750) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameALDH6A1 Rabbit pAb
Catalog No.A14750
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the aldehyde dehydrogenase protein family. The encoded protein is a mitochondrial methylmalonate semialdehyde dehydrogenase that plays a role in the valine and pyrimidine catabolic pathways. This protein catalyzes the irreversible oxidative decarboxylation of malonate and methylmalonate semialdehydes to acetyl- and propionyl-CoA. Methylmalonate semialdehyde dehydrogenase deficiency is characterized by elevated beta-alanine, 3-hydroxypropionic acid, and both isomers of 3-amino and 3-hydroxyisobutyric acids in urine organic acids. Alternate splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 326-535 of human ALDH6A1 (NP_005580.1).
SequenceVGEAKKWLPELVEHAKNLRVNAGDQPGADLGPLITPQAKERVCNLIDSGTKEGASILLDGRKIKVKGYENGNFVGPTIISNVKPNMTCYKEEIFGPVLVVLETETLDEAIQIVNNNPYGNGTAIFTTNGATARKYAHLVDVGQVGVNVPIPVPLPMFSFTGSRSSFRGDTNFYGKQGIQFYTQLKTITSQWKEEDATLSSPAVVMPTMGR
Gene ID
Swiss Prot
SynonymsMMSDH; MMSADHA; ALDH6A1
Calculated MW58kDa
Observed MW
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationImmunohistochemistry    Immunofluorescence    
Positive samples
Cellular locationMitochondrion

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ABclonal:Immunohistochemistry - ALDH6A1 Rabbit pAb (A14750)}

Immunohistochemistry - ALDH6A1 Rabbit pAb (A14750)

Immunohistochemistry analysis of paraffin-embedded rat ovary using ALDH6A1 Rabbit pAb (A14750) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - ALDH6A1 Rabbit pAb (A14750)}

Immunohistochemistry - ALDH6A1 Rabbit pAb (A14750)

Immunohistochemistry analysis of paraffin-embedded human stomach using ALDH6A1 Rabbit pAb (A14750) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - ALDH6A1 Rabbit pAb (A14750)}

Immunohistochemistry - ALDH6A1 Rabbit pAb (A14750)

Immunohistochemistry analysis of paraffin-embedded mouse kidney using ALDH6A1 Rabbit pAb (A14750) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - ALDH6A1 Rabbit pAb (A14750)}

Immunofluorescence - ALDH6A1 Rabbit pAb (A14750)

Immunofluorescence analysis of HeLa cells using ALDH6A1 Rabbit pAb (A14750) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ALDH6A1 Rabbit pAb (A14750)}

Immunofluorescence - ALDH6A1 Rabbit pAb (A14750)

Immunofluorescence analysis of NIH-3T3 cells using ALDH6A1 Rabbit pAb (A14750) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (1)

Secondary Antibodies (26)