제품 > 항체 > 다중클론항체(pAb)

ALDH9A1 Rabbit pAb (A7875)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ALDH9A1 Rabbit pAb (A7875)

Western blot analysis of various lysates, using ALDH9A1 Rabbit pAb (A7875) at 1:1500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - ALDH9A1 Rabbit pAb (A7875)

Western blot analysis of lysates from U-251 MG cells using ALDH9A1 Rabbit pAb (A7875) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Overview

Product nameALDH9A1 Rabbit pAb
Catalog No.A7875
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This protein belongs to the aldehyde dehydrogenase family of proteins. It has a high activity for oxidation of gamma-aminobutyraldehyde and other amino aldehydes. The enzyme catalyzes the dehydrogenation of gamma-aminobutyraldehyde to gamma-aminobutyric acid (GABA). This isozyme is a tetramer of identical 54-kD subunits.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-180 of human ALDH9A1 (NP_000687.3).
SequenceMFLRAGLAALSPLLRSLRPSPVAAMSTGTFVVSQPLNYRGGARVEPADASGTEKAFEPATGRVIATFTCSGEKEVNLAVQNAKAAFKIWSQKSGMERCRILLEAARIIREREDEIATMECINNGKSIFEARLDIDISWQCLEYYAGLAASMAGEHIQLPGGSFGYTRREPLGVCVGIGAW
Gene ID
Swiss Prot
SynonymsE3; ALDH4; ALDH7; ALDH9; TMABADH; TMABA-DH; TMABALDH; ALDH9A1
Calculated MW54kDa
Observed MW54kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesDaudi(Negative control), Mouse liver, Rat kidney, Rat liver, U-251 MG
Cellular locationCytoplasm.

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ABclonal:Western blot - ALDH9A1 Rabbit pAb (A7875)}

Western blot - ALDH9A1 Rabbit pAb (A7875)

Western blot analysis of various lysates, using ALDH9A1 Rabbit pAb (A7875) at 1:1500 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Western blot - ALDH9A1 Rabbit pAb (A7875)}

Western blot - ALDH9A1 Rabbit pAb (A7875)

Western blot analysis of lysates from U-251 MG cells using ALDH9A1 Rabbit pAb (A7875) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

* For research use only. Not for therapeutic or diagnostic purposes.

Secondary Antibodies (26)