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ALKBH1 Rabbit mAb (A9221)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ALKBH1 Rabbit mAb (A9221)

Western blot analysis of various lysates, using ALKBH1 Rabbit mAb (A9221) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - ALKBH1 Rabbit mAb (A9221)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using ALKBH1 Rabbit mAb (A9221) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ALKBH1 Rabbit mAb (A9221)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using ALKBH1 Rabbit mAb (A9221) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - ALKBH1 Rabbit mAb (A9221)

Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using ALKBH1 Rabbit mAb (A9221) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product nameALKBH1 Rabbit mAb
Catalog No.A9221
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2511
This gene encodes a homolog to the E. coli alkB gene product. The E. coli alkB protein is part of the adaptive response mechanism of DNA alkylation damage repair. It is involved in damage reversal by oxidative demethylation of 1-methyladenine and 3-methylcytosine.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human ALKBH1 (Q13686).
SequenceMGKMAAAVGSVATLATEPGEDAFRKLFRFYRQSRPGTADLEGVIDFSAAHAARGKGPGAQKVIKSQLNVSSVSEQNAYRAGLQPVSKWQAYGLKGYPGFI
Gene ID
Swiss Prot
SynonymsABH; ABH1; alkB; hABH; ALKBH; ALKBH1
Calculated MW44kDa
Observed MW44kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:6000
  • IHC-P 1:200 - 1:2000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samples293T, PC-12, A549, K-562
Cellular locationMitochondrion, Nucleus.

Documents

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ABclonal:Western blot - ALKBH1 Rabbit mAb (A9221)}

Western blot - ALKBH1 Rabbit mAb (A9221)

Western blot analysis of various lysates, using ALKBH1 Rabbit mAb (A9221) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - ALKBH1 Rabbit mAb (A9221)}

Immunohistochemistry - ALKBH1 Rabbit mAb (A9221)

Immunohistochemistry analysis of paraffin-embedded Human liver tissue using ALKBH1 Rabbit mAb (A9221) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ALKBH1 Rabbit mAb (A9221)}

Immunohistochemistry - ALKBH1 Rabbit mAb (A9221)

Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using ALKBH1 Rabbit mAb (A9221) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - ALKBH1 Rabbit mAb (A9221)}

Immunohistochemistry - ALKBH1 Rabbit mAb (A9221)

Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using ALKBH1 Rabbit mAb (A9221) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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