제품 > 항체 > 다중클론항체(pAb)

AMD1 Rabbit pAb (A8537)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - AMD1 Rabbit pAb (A8537)

Western blot analysis of extracts of Mouse skeletal muscle, using AMD1 antibody (A8537) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Western blot - AMD1 Rabbit pAb (A8537)

Western blot analysis of extracts of various cell lines, using AMD1 antibody (A8537) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product nameAMD1 Rabbit pAb
Catalog No.A8537
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an important intermediate enzyme in polyamine biosynthesis. The polyamines spermine, spermidine, and putrescine are low-molecular-weight aliphatic amines essential for cellular proliferation and tumor promotion. Multiple alternatively spliced transcript variants have been identified. Pseudogenes of this gene are found on chromosomes 5, 6, 10, X and Y.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 75-334 of human AMD1 (NP_001625.2).
SequenceRRFILKTCGTTLLLKALVPLLKLARDYSGFDSIQSFFYSRKNFMKPSHQGYPHRNFQEEIEFLNAIFPNGAAYCMGRMNSDCWYLYTLDFPESRVISQPDQTLEILMSELDPAVMDQFYMKDGVTAKDVTRESGIRDLIPGSVIDATMFNPCGYSMNGMKSDGTYWTIHITPEPEFSYVSFETNLSQTSYDDLIRKVVEVFKPGKFVTTLFVNQSSKCRTVLASPQKIEGFKRLDCQSAMFNDYNFVFTSFAKKQQQQQS
Gene ID
Swiss Prot
SynonymsAMD; SAMDC; ADOMETDC
Calculated MW38kDa
Observed MW32kDa/42kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samples293T, Mouse skeletal muscle, Rat testis
Cellular locationcytosol

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - AMD1 Rabbit pAb (A8537)}

    Western blot - AMD1 Rabbit pAb (A8537)

    Western blot analysis of extracts of Mouse skeletal muscle, using AMD1 antibody (A8537) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 60s.
    ABclonal:Western blot - AMD1 Rabbit pAb (A8537)}

    Western blot - AMD1 Rabbit pAb (A8537)

    Western blot analysis of extracts of various cell lines, using AMD1 antibody (A8537) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Secondary Antibodies (26)