제품 > 항체 > 다중클론항체(pAb)

ANGEL2 Rabbit pAb (A12799)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - ANGEL2 Rabbit pAb (A12799)

Western blot analysis of various lysates using ANGEL2 Rabbit pAb (A12799) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

Overview

Product nameANGEL2 Rabbit pAb
Catalog No.A12799
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Enables mRNA 3'-UTR binding activity. Involved in 3'-UTR-mediated mRNA stabilization and negative regulation of mitotic cell cycle. Located in Cajal body and cytoplasm.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 30-170 of human ANGEL2 (NP_653168.2).
SequenceSLGRDWTTPWENLQRCCWNRHISSCMRWPGHYSRAPYPYFSSRHFSLNWRPPCLFESRTQFQYCNWRPDNLSQTSLIHLSSYVMNAEGDEPSSKRRKHQGVIKRNWEYICSHDKEKTKILGDKNVDPKCEDSENKFDFSVM
Gene ID
Swiss Prot
SynonymsCcr4d; KIAA0759L; ANGEL2
Calculated MW62kDa
Observed MW62kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesU-87MG, U-251MG
Cellular locationCajal body, cytoplasm

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    ABclonal:Western blot - ANGEL2 Rabbit pAb (A12799)}

    Western blot - ANGEL2 Rabbit pAb (A12799)

    Western blot analysis of various lysates using ANGEL2 Rabbit pAb (A12799) at 1:3000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 90s.

    * For research use only. Not for therapeutic or diagnostic purposes.