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ANO2 Rabbit pAb (A13109)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ANO2 Rabbit pAb (A13109)

Western blot analysis of lysates from HeLa cells, using ANO2 Rabbit pAb (A13109) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 20s.

ABclonal:Immunohistochemistry - ANO2 Rabbit pAb (A13109)

Immunohistochemistry analysis of paraffin-embedded rat retina using ANO2 Rabbit pAb (A13109) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ANO2 Rabbit pAb (A13109)

Immunohistochemistry analysis of paraffin-embedded mouse retina using ANO2 Rabbit pAb (A13109) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Overview

Product nameANO2 Rabbit pAb
Catalog No.A13109
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ANO2 belongs to a family of calcium-activated chloride channels (CaCCs) (reviewed by Hartzell et al., 2009 [PubMed 19015192]).
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human ANO2 (NP_001265526.1).
SequenceMPEDIPLLPGSPRRLSPQAGSRGGQGPKHGQQCLKMPGPRAPGLQGGSNRDPGQPCGGESTRSSSVINNYLDANEPVSLEARLSRMHFHDSQRKVDYVLAYHYRKRGVHLAQGFPGHSLAIVSNGETGKEPHAGGPGDIELGPLDALEEERKEQREEFEHNLMEAGLELEKDLENKSQGSIFVRIHAPWQVLAREAEFLK
Gene ID
Swiss Prot
SynonymsC12orf3; TMEM16B; ANO2
Calculated MW114kDa
Observed MW114kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:2000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa
Cellular locationCell membrane, Multi-pass membrane protein

Documents

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    ABclonal:Western blot - ANO2 Rabbit pAb (A13109)}

    Western blot - ANO2 Rabbit pAb (A13109)

    Western blot analysis of lysates from HeLa cells, using ANO2 Rabbit pAb (A13109) at 1:3000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 20s.
    ABclonal:Immunohistochemistry - ANO2 Rabbit pAb (A13109)}

    Immunohistochemistry - ANO2 Rabbit pAb (A13109)

    Immunohistochemistry analysis of paraffin-embedded rat retina using ANO2 Rabbit pAb (A13109) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
    ABclonal:Immunohistochemistry - ANO2 Rabbit pAb (A13109)}

    Immunohistochemistry - ANO2 Rabbit pAb (A13109)

    Immunohistochemistry analysis of paraffin-embedded mouse retina using ANO2 Rabbit pAb (A13109) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.

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