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AP3S2 Rabbit pAb (A17607)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - AP3S2 Rabbit pAb (A17607)

Western blot analysis of various lysates using AP3S2 Rabbit pAb (A17607) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - AP3S2 Rabbit pAb (A17607)

Immunofluorescence analysis of C6 cells using AP3S2 Rabbit pAb (A17607) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - AP3S2 Rabbit pAb (A17607)

Immunofluorescence analysis of U-2 OS cells using AP3S2 Rabbit pAb (A17607) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameAP3S2 Rabbit pAb
Catalog No.A17607
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Predicted to be involved in anterograde synaptic vesicle transport and vesicle-mediated transport. Located in intracellular membrane-bounded organelle. Part of AP-3 adaptor complex.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human AP3S2 (NP_005820.1).
SequenceMIQAILVFNNHGKPRLVRFYQRFPEEIQQQIVRETFHLVLKRDDNICNFLEGGSLIGGSDYKLIYRHYATLYFVFCVDSSESELGILDLIQVFVETLDKCFENVCELDLIFHMDKVHYILQEVVMGGMVLETNMNEIVAQIEAQNRLEKSEGGLSAAPARAVSAVKNINLPEIPRNINIGDLNIKVPNLSQFV
Gene ID
Swiss Prot
SynonymsAP3S3; sigma3b; AP3S2
Calculated MW22kDa
Observed MW22kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesHT-29, Mouse brain, Rat brain
Cellular locationaxon cytoplasm, early endosome, Golgi apparatus, presynapse

Documents

Certificate of Compliance

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    ABclonal:Western blot - AP3S2 Rabbit pAb (A17607)}

    Western blot - AP3S2 Rabbit pAb (A17607)

    Western blot analysis of various lysates using AP3S2 Rabbit pAb (A17607) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunofluorescence - AP3S2 Rabbit pAb (A17607)}

    Immunofluorescence - AP3S2 Rabbit pAb (A17607)

    Immunofluorescence analysis of C6 cells using AP3S2 Rabbit pAb (A17607) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - AP3S2 Rabbit pAb (A17607)}

    Immunofluorescence - AP3S2 Rabbit pAb (A17607)

    Immunofluorescence analysis of U-2 OS cells using AP3S2 Rabbit pAb (A17607) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.