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APLP1 Rabbit pAb (A6870)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - APLP1 Rabbit pAb (A6870)

Western blot analysis of various lysates using APLP1 Rabbit pAb (A6870) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - APLP1 Rabbit pAb (A6870)

Immunohistochemistry analysis of paraffin-embedded Rat brain using APLP1 Rabbit pAb (A6870) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunohistochemistry - APLP1 Rabbit pAb (A6870)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using APLP1 Rabbit pAb (A6870) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - APLP1 Rabbit pAb (A6870)

Immunofluorescence analysis of Neuro-2a cells using APLP1 Rabbit pAb (A6870) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - APLP1 Rabbit pAb (A6870)

Immunofluorescence analysis of SH-SY5Y cells using APLP1 Rabbit pAb (A6870) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameAPLP1 Rabbit pAb
Catalog No.A6870
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the highly conserved amyloid precursor protein gene family. The encoded protein is a membrane-associated glycoprotein that is cleaved by secretases in a manner similar to amyloid beta A4 precursor protein cleavage. This cleavage liberates an intracellular cytoplasmic fragment that may act as a transcriptional activator. The encoded protein may also play a role in synaptic maturation during cortical development. Alternatively spliced transcript variants encoding different isoforms have been described.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 285-490 of human APLP1 (NP_005157.1).
SequenceTPTPRPTDGVDIYFGMPGEISEHEGFLRAKMDLEERRMRQINEVMREWAMADNQSKNLPKADRQALNEHFQSILQTLEEQVSGERQRLVETHATRVIALINDQRRAALEGFLAALQADPPQAERVLLALRRYLRAEQKEQRHTLRHYQHVAAVDPEKAQQMRFQVHTHLQVIEERVNQSLGLLDQNPHLAQELRPQIQELLHSEHL
Gene ID
Swiss Prot
SynonymsAPLP; APLP1
Calculated MW72kDa
Observed MW72kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse brain, Rat brain
Cellular locationCell membrane, Cytoplasm, Single-pass type I membrane protein.

Documents

Certificate of Compliance

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ABclonal:Western blot - APLP1 Rabbit pAb (A6870)}

Western blot - APLP1 Rabbit pAb (A6870)

Western blot analysis of various lysates using APLP1 Rabbit pAb (A6870) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - APLP1 Rabbit pAb (A6870)}

Immunohistochemistry - APLP1 Rabbit pAb (A6870)

Immunohistochemistry analysis of paraffin-embedded Rat brain using APLP1 Rabbit pAb (A6870) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunohistochemistry - APLP1 Rabbit pAb (A6870)}

Immunohistochemistry - APLP1 Rabbit pAb (A6870)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using APLP1 Rabbit pAb (A6870) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
ABclonal:Immunofluorescence - APLP1 Rabbit pAb (A6870)}

Immunofluorescence - APLP1 Rabbit pAb (A6870)

Immunofluorescence analysis of Neuro-2a cells using APLP1 Rabbit pAb (A6870) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - APLP1 Rabbit pAb (A6870)}

Immunofluorescence - APLP1 Rabbit pAb (A6870)

Immunofluorescence analysis of SH-SY5Y cells using APLP1 Rabbit pAb (A6870) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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