제품 > 항체 > 다중클론항체(pAb)

APPBP2 Rabbit pAb (A15781)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - APPBP2 Rabbit pAb (A15781)

Western blot analysis of various lysates using APPBP2 Rabbit pAb (A15781) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 35s.

Overview

Product nameAPPBP2 Rabbit pAb
Catalog No.A15781
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene interacts with microtubules and is functionally associated with beta-amyloid precursor protein transport and/or processing. The beta-amyloid precursor protein is a cell surface protein with signal-transducing properties, and it is thought to play a role in the pathogenesis of Alzheimer's disease. The encoded protein may be involved in regulating cell death. This gene has been found to be highly expressed in breast cancer. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 386-585 of human APPBP2 (NP_006371.2).
SequenceLEEIAIDCHNKETEQRLLQEAHDLHLSSLQLAKKAFGEFNVQTAKHYGNLGRLYQSMRKFKEAEEMHIKAIQIKEQLLGQEDYEVALSVGHLASLYNYDMNQYENAEKLYLRSIAIGKKLFGEGYSGLEYDYRGLIKLYNSIGNYEKVFEYHNVLSNWNRLRDRQYSVTDALEDVSTSPQSTEEVVQSFLISQNVEGPSC
Gene ID
Swiss Prot
SynonymsPAT1; APP-BP2; HS.84084; APPBP2
Calculated MW67kDa
Observed MW67kDa, 71kDa, 80kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesA-431, HeLa, Jurkat, Raji, Mouse kidney, Mouse brain, Rat kidney, Rat brain
Cellular locationCytoplasm, Membrane, Nucleus, Peripheral membrane protein, cytoskeleton

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ABclonal:Western blot - APPBP2 Rabbit pAb (A15781)}

Western blot - APPBP2 Rabbit pAb (A15781)

Western blot analysis of various lysates using APPBP2 Rabbit pAb (A15781) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 35s.

* For research use only. Not for therapeutic or diagnostic purposes.