제품 > 항체 > 다중클론항체(pAb)

APRR3 Rabbit pAb (A22254)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Arabidopsis thaliana

ABclonal:Western blot - APRR3 Rabbit pAb (A22254)

Western blot analysis of extracts of various tissues from Arabidopsis thaliana, using APRR3 Rabbit pAb (A22254) at 1:900 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

Overview

Product nameAPRR3 Rabbit pAb
Catalog No.A22254
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Encodes pseudo-response regulator 3 (APRR3/PRR3). PRR3 transcript levels vary in a circadian pattern with peak expression at dusk under long and short day conditions. PRR3 affects the period of the circadian clock and seedlings with reduced levels of PRR3 have shorter periods, based on transcriptional assays of clock-regulated genes. PRR3 is expressed in the vasculature of cotyledons and leaves where it may help stabilize the TOC1 protein by preventing interactions between TOC1 and the F-box protein ZTL.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 245-371 of Arabidopsis thaliana APRR3 (NP_568919.1).
SequenceTQSSWTKRASDTKSTSPSNQFPDAPNKKGTYENGCAHVNRLKEAEDQKEQIGTGSQTGMSMSKKAEEPGDLEKNAKYSVQALERNNDDTLNRSSGNSQVESKAPSSNREDLQSLEQTLKKTREDRDY
Gene ID
Swiss Prot
SynonymsAPRR3; MGO3.8; MGO3_8; pseudo-response regulator 3
Calculated MW
Observed MW55kDa
ReactivityArabidopsis thaliana
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesSeedling, Before inflorescence, Rosette leaf
Cellular location

Documents

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    ABclonal:Western blot - APRR3 Rabbit pAb (A22254)}

    Western blot - APRR3 Rabbit pAb (A22254)

    Western blot analysis of extracts of various tissues from Arabidopsis thaliana, using APRR3 Rabbit pAb (A22254) at 1:900 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 20s.

    * For research use only. Not for therapeutic or diagnostic purposes.