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A-RAF Rabbit mAb (A8687)

Publication (1) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Western blot - A-RAF Rabbit mAb (A8687)

Western blot analysis of various lysates using A-RAF Rabbit mAb (A8687) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - A-RAF Rabbit mAb (A8687)

Immunohistochemistry analysis of paraffin-embedded Human liver using A-RAF Rabbit mAb (A8687) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Overview

Product nameA-RAF Rabbit mAb
Catalog No.A8687
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC1782
Enables protein serine/threonine kinase activity. Involved in negative regulation of apoptotic process; regulation of TOR signaling; and regulation of cellular protein metabolic process. Predicted to be active in cytosol and mitochondrion. Biomarker of high grade glioma.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 166-290 of human A-RAF (P10398).
SequenceRQHEAPSNRPLNELLTPQGPSPRTQHCDPEHFPFPAPANAPLQRIRSTSTPNVHMVSTTAPMDSNLIQLTGQSFSTDAAGSRGGSDGTPRGSPSPASVSSGRKSPHSKSPAEQRERKSLADDKKK
Gene ID
Swiss Prot
SynonymsPKS2; A-RAF; ARAF1; RAFA1
Calculated MW68kDa
Observed MW68kDa
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesHeLa, MCF7
Cellular locationCytosol, Mitochondrion
Customer validation

WB(Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - A-RAF Rabbit mAb (A8687)}

Western blot - A-RAF Rabbit mAb (A8687)

Western blot analysis of various lysates using A-RAF Rabbit mAb (A8687) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - A-RAF Rabbit mAb (A8687)}

Immunohistochemistry - A-RAF Rabbit mAb (A8687)

Immunohistochemistry analysis of paraffin-embedded Human liver using A-RAF Rabbit mAb (A8687) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

* For research use only. Not for therapeutic or diagnostic purposes.

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