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ARF6 Rabbit pAb (A0988)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ARF6 Rabbit pAb (A0988)

Western blot analysis of extracts of various cell lines, using ARF6 antibody (A0988) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 10s.

ABclonal:Immunofluorescence - ARF6 Rabbit pAb (A0988)

Immunofluorescence analysis of HeLa cells using ARF6 antibody (A0988) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameARF6 Rabbit pAb
Catalog No.A0988
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-175 of human ARF6 (NP_001654.1).
SequenceMGKVLSKIFGNKEMRILMLGLDAAGKTTILYKLKLGQSVTTIPTVGFNVETVTYKNVKFNVWDVGGQDKIRPLWRHYYTGTQGLIFVVDCADRDRIDEARQELHRIINDREMRDAIILIFANKQDLPDAMKPHEIQEKLGLTRIRDRNWYVQPSCATSGDGLYEGLTWLTSNYKS
Gene ID
Swiss Prot
SynonymsARF6
Calculated MW20kDa
Observed MW19kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesU-87MG, DU145, Mouse brain, Rat brain, Rat spleen
Cellular locationCell membrane, Cell projection, Cleavage furrow, Cytoplasm, Endosome membrane, Golgi apparatus, Lipid-anchor, Midbody, Recycling endosome membrane, filopodium membrane
Customer validation

IHC(Homo sapiens,Mus musculus)

WB(Homo sapiens,Mus musculus)

Documents

Certificate of Compliance

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ABclonal:Western blot - ARF6 Rabbit pAb (A0988)}

Western blot - ARF6 Rabbit pAb (A0988)

Western blot analysis of extracts of various cell lines, using ARF6 antibody (A0988) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 10s.
ABclonal:Immunofluorescence - ARF6 Rabbit pAb (A0988)}

Immunofluorescence - ARF6 Rabbit pAb (A0988)

Immunofluorescence analysis of HeLa cells using ARF6 antibody (A0988) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using A0988? Please let us know so that we can cite the reference in this datasheet.

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