제품 > 항체 > 다중클론항체(pAb)

ARHGAP1 Rabbit pAb (A3739)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using ARHGAP1 Rabbit pAb (A3739) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.

ABclonal:Immunoprecipitation - ARHGAP1 Rabbit pAb (A3739)

Immunoprecipitation analysis of 200 μg extracts of HeLa cells, using 3 μg ARHGAP1 antibody (A3739). Western blot was performed from the immunoprecipitate using ARHGAP1 antibody (A3739) at a dilution of 1:1000.

Overview

Product name	ARHGAP1 Rabbit pAb
Catalog No.	A3739
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a member of a large family of proteins that activate Rho-type guanosine triphosphate (GTP) metabolizing enzymes. The encoded protein contains a SRC homology 3 domain and interacts with Bcl-2-associated protein family members.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 210-439 of human ARHGAP1 (NP_004299.1).
Sequence	VLKYDDFLKSTQKSPATAPKPMPPRPPLPNQQFGVSLQHLQEKNPEQEPIPIVLRETVAYLQAHALTTEGIFRRSANTQVVREVQQKYNMGLPVDFDQYNELHLPAVILKTFLRELPEPLLTFDLYPHVVGFLNIDESQRVPATLQVLQTLPEENYQVLRFLTAFLVQISAHSDQNKMTNTNLAVVFGPNLLWAKDAAITLKAINPINTFTKFLLDHQGELFPSPDPSGL
Gene ID
Swiss Prot
Synonyms	RHOGAP; RHOGAP1; CDC42GAP; p50rhoGAP; ARHGAP1
Calculated MW	50kDa
Observed MW	50kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting Immunoprecipitation
Positive samples	BxPC-3, HeLa, HepG2, U-87MG, PC-12, Mouse lung
Cellular location	Cytoplasm