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ARHGAP4 Rabbit pAb (A18338)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ARHGAP4 Rabbit pAb (A18338)

Western blot analysis of lysates from Jurkat cells, using ARHGAP4 Rabbit pAb (A18338) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - ARHGAP4 Rabbit pAb (A18338)

Immunohistochemistry analysis of paraffin-embedded rat heart using ARHGAP4 Rabbit pAb (A18338) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ARHGAP4 Rabbit pAb (A18338)

Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using ARHGAP4 Rabbit pAb (A18338) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ARHGAP4 Rabbit pAb (A18338)

Immunohistochemistry analysis of paraffin-embedded mouse heart using ARHGAP4 Rabbit pAb (A18338) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - ARHGAP4 Rabbit pAb (A18338)

Immunofluorescence analysis of L929 cells using ARHGAP4 Rabbit pAb (A18338) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameARHGAP4 Rabbit pAb
Catalog No.A18338
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the rhoGAP family of proteins which play a role in the regulation of small GTP-binding proteins belonging to the RAS superfamily. The protein encoded by the orthologous gene in rat is localized to the Golgi complex and can redistribute to microtubules. The rat protein stimulates the activity of some Rho GTPases in vitro. Genomic deletions of this gene and a neighboring gene have been found in patients with nephrogenic diabetes insipidus. Multiple transcript variants encoding different isoforms have been found for this gene.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 900 to the C-terminus of human ARHGAP4 (NP_001158213.1).
SequenceSPEQHVEVDKAVAQNMDSVFKELLGKTSVRQGLGPASTTSPSPGPRSPKAPPSSRLGRNKGFSRGPGAPASPSASHPQGLDTTPKPH
Gene ID
Swiss Prot
SynonymsC1; RGC1; p115; SrGAP4; RhoGAP4; ARHGAP4
Calculated MW105kDa
Observed MW105kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesJurkat
Cellular locationcytoplasm, cytosol, Golgi apparatus, nucleoplasm

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ABclonal:Western blot - ARHGAP4 Rabbit pAb (A18338)}

Western blot - ARHGAP4 Rabbit pAb (A18338)

Western blot analysis of lysates from Jurkat cells, using ARHGAP4 Rabbit pAb (A18338) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - ARHGAP4 Rabbit pAb (A18338)}

Immunohistochemistry - ARHGAP4 Rabbit pAb (A18338)

Immunohistochemistry analysis of paraffin-embedded rat heart using ARHGAP4 Rabbit pAb (A18338) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - ARHGAP4 Rabbit pAb (A18338)}

Immunohistochemistry - ARHGAP4 Rabbit pAb (A18338)

Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using ARHGAP4 Rabbit pAb (A18338) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - ARHGAP4 Rabbit pAb (A18338)}

Immunohistochemistry - ARHGAP4 Rabbit pAb (A18338)

Immunohistochemistry analysis of paraffin-embedded mouse heart using ARHGAP4 Rabbit pAb (A18338) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - ARHGAP4 Rabbit pAb (A18338)}

Immunofluorescence - ARHGAP4 Rabbit pAb (A18338)

Immunofluorescence analysis of L929 cells using ARHGAP4 Rabbit pAb (A18338) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.