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ARID1A Rabbit mAb (A19570)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ARID1A Rabbit mAb (A19570)

Western blot analysis of lysates from PC-3 cells, using ARID1A Rabbit mAb (A19570) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - ARID1A Rabbit mAb (A19570)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using ARID1A Rabbit mAb (A19570) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - ARID1A Rabbit mAb (A19570)

Immunohistochemistry analysis of paraffin-embedded rat ovary using ARID1A Rabbit mAb (A19570) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - ARID1A Rabbit mAb (A19570)

Confocal imaging of NIH-3T3 cells using ARID1A Rabbit mAb (A19570, dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

ABclonal:Immunofluorescence - ARID1A Rabbit mAb (A19570)

Confocal imaging of U-2 OS cells using ARID1A Rabbit mAb (A19570, dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Overview

Product nameARID1A Rabbit mAb
Catalog No.A19570
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC2177
This gene encodes a member of the SWI/SNF family, whose members have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. It possesses at least two conserved domains that could be important for its function. First, it has a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. Second, the C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that the protein encoded by this gene confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions. Two transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1204-1314 of human ARID1A (O14497).
SequenceSMTPNPGYQPSMNTSDMMGRMSYEPNKDPYGSMRKAPGSDPFMSSGQGPNGGMGDPYSRAAGPGLGNVAMGPRQHYPYGGPYDRVRTEPGIGPEGNMSTGAPQPNLMPSNP
Gene ID
Swiss Prot
SynonymsELD; B120; CSS2; OSA1; P270; hELD; BM029; MRD14; hOSA1; BAF250; C1orf4; BAF250a; SMARCF1; ARID1A
Calculated MW242kDa
Observed MW270kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesPC-3
Cellular locationnucleoplasm, nucleus

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ABclonal:Western blot - ARID1A Rabbit mAb (A19570)}

Western blot - ARID1A Rabbit mAb (A19570)

Western blot analysis of lysates from PC-3 cells, using ARID1A Rabbit mAb (A19570) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - ARID1A Rabbit mAb (A19570)}

Immunohistochemistry - ARID1A Rabbit mAb (A19570)

Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using ARID1A Rabbit mAb (A19570) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - ARID1A Rabbit mAb (A19570)}

Immunohistochemistry - ARID1A Rabbit mAb (A19570)

Immunohistochemistry analysis of paraffin-embedded rat ovary using ARID1A Rabbit mAb (A19570) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - ARID1A Rabbit mAb (A19570)}

Immunofluorescence - ARID1A Rabbit mAb (A19570)

Confocal imaging of NIH-3T3 cells using ARID1A Rabbit mAb (A19570, dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
ABclonal:Immunofluorescence - ARID1A Rabbit mAb (A19570)}

Immunofluorescence - ARID1A Rabbit mAb (A19570)

Confocal imaging of U-2 OS cells using ARID1A Rabbit mAb (A19570, dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

* For research use only. Not for therapeutic or diagnostic purposes.

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