제품 > 항체 > 다중클론항체(pAb)

ARL1 Rabbit pAb (A15254)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ARL1 Rabbit pAb (A15254)

Western blot analysis of various lysates using ARL1 Rabbit pAb (A15254) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Immunofluorescence - ARL1 Rabbit pAb (A15254)

Immunofluorescence analysis of C6 cells using ARL1 Rabbit pAb (A15254) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - ARL1 Rabbit pAb (A15254)

Immunofluorescence analysis of L929 cells using ARL1 Rabbit pAb (A15254) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameARL1 Rabbit pAb
Catalog No.A15254
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the ARL (ADP-ribosylation factor-like) family of proteins, which are structurally related to ADP-ribosylation factors (ARFs). ARFs, described as activators of cholera toxin (CT) ADP-ribosyltransferase activity, regulate intracellular vesicular membrane trafficking, and stimulate a phospholipase D (PLD) isoform. Although, ARL proteins were initially thought not to activate CT or PLD, later work showed that they are weak stimulators of PLD and CT in a phospholipid dependent manner. Alternative splicing results in multiple transcript variants encoding different isoforms.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 62-181 of human ARL1 (NP_001168.1).
SequenceKFQVWDLGGQTSIRPYWRCYYSNTDAVIYVVDSCDRDRIGISKSELVAMLEEEELRKAILVVFANKQDMEQAMTSSEMANSLGLPALKDRKWQIFKTSATKGTGLDEAMEWLVETLKSRQ
Gene ID
Swiss Prot
SynonymsARFL1; ARL1
Calculated MW20kDa
Observed MW20kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:200 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samples293T, A-549, Mouse thymus, Mouse brain
Cellular locationCytoplasmic side, Golgi apparatus membrane, Lipid-anchor, Membrane, Peripheral membrane protein

Documents

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    ABclonal:Western blot - ARL1 Rabbit pAb (A15254)}

    Western blot - ARL1 Rabbit pAb (A15254)

    Western blot analysis of various lysates using ARL1 Rabbit pAb (A15254) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 3min.
    ABclonal:Immunofluorescence - ARL1 Rabbit pAb (A15254)}

    Immunofluorescence - ARL1 Rabbit pAb (A15254)

    Immunofluorescence analysis of C6 cells using ARL1 Rabbit pAb (A15254) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - ARL1 Rabbit pAb (A15254)}

    Immunofluorescence - ARL1 Rabbit pAb (A15254)

    Immunofluorescence analysis of L929 cells using ARL1 Rabbit pAb (A15254) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.