제품 > 항체 > 다중클론항체(pAb)

ARMC6 Rabbit pAb (A15931)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ARMC6 Rabbit pAb (A15931)

Western blot analysis of various lysates using ARMC6 Rabbit pAb (A15931) at 1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - ARMC6 Rabbit pAb (A15931)

Immunofluorescence analysis of L929 cells using ARMC6 Rabbit pAb (A15931) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameARMC6 Rabbit pAb
Catalog No.A15931
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The function of this gene's protein product has not been determined. A related protein in mouse suggests that this protein has a conserved function. Two transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human ARMC6 (NP_001186125.1).
SequenceMSERCCSRYSSGASIGCTPTSTQAKMVSKRIAQETFDAAVRENIEEFAMGPEEAVKEAVEQFESQGVDLSNIVKTAPKVSADGSQEPTHDILQMLSDLQESVASSRPQEVSAYLTRFCDQCKQDKACRFLAAQKGAYPIIFTAWKLATAGDQGLLLQSLNALSVLTDGQPDLLDAQGLQLLVATLTQNADEADLTCSGIRCVRHACLKHEQNRQDLVKAG
Gene ID
Swiss Prot
SynonymsR30923_1; ARMC6
Calculated MW54kDa
Observed MW54kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesA-431, 293T, LO2, Mouse brain, Mouse liver, Mouse pancreas, Rat testis, Rat brain
Cellular locationcytosol

Documents

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    ABclonal:Western blot - ARMC6 Rabbit pAb (A15931)}

    Western blot - ARMC6 Rabbit pAb (A15931)

    Western blot analysis of various lysates using ARMC6 Rabbit pAb (A15931) at 1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunofluorescence - ARMC6 Rabbit pAb (A15931)}

    Immunofluorescence - ARMC6 Rabbit pAb (A15931)

    Immunofluorescence analysis of L929 cells using ARMC6 Rabbit pAb (A15931) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.