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ASAH1 Rabbit pAb (A13948)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - ASAH1 Rabbit pAb (A13948)

Western blot analysis of lysates from RT4 cells using ASAH1 Rabbit pAb(A13948) at 1:700 dilution.
Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection:ECL Basic Kit (RM00020).
Exposuretime:60s.

ABclonal:Immunofluorescence - ASAH1 Rabbit pAb (A13948)

Immunofluorescence analysis of SH-SY5Y cells using ASAH1 Rabbit pAb (A13948) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameASAH1 Rabbit pAb
Catalog No.A13948
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the acid ceramidase family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. Processing of this preproprotein generates alpha and beta subunits that heterodimerize to form the mature lysosomal enzyme, which catalyzes the degradation of ceramide into sphingosine and free fatty acid. This enzyme is overexpressed in multiple human cancers and may play a role in cancer progression. Mutations in this gene are associated with the lysosomal storage disorder, Farber lipogranulomatosis, and a neuromuscular disorder, spinal muscular atrophy with progressive myoclonic epilepsy.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 140-389 of human ASAH1 (NP_001120977.1).
SequenceIVAEDKKGHLIHGRNMDFGVFLGWNINNDTWVITEQLKPLTVNLDFQRNNKTVFKASSFAGYVGMLTGFKPGLFSLTLNERFSINGGYLGILEWILGKKDVMWIGFLTRTVLENSTSYEEAKNLLTKTKILAPAYFILGGNQSGEGCVITRDRKESLDVYELDAKQGRWYVVQTNYDRWKHPFFLDDRRTPAKMCLNRTSQENISFETMYDVLSTKPVLNKLTVYTTLIDVTKGQFETYLRDCPDPCIGW
Gene ID
Swiss Prot
SynonymsAC; PHP; ASAH; PHP32; ACDase; SMAPME
Calculated MW45kDa
Observed MW40kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunofluorescence    
Positive samplesRT4
Cellular locationLysosome

Documents

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    ABclonal:Western blot - ASAH1 Rabbit pAb (A13948)}

    Western blot - ASAH1 Rabbit pAb (A13948)

    Western blot analysis of lysates from RT4 cells using ASAH1 Rabbit pAb(A13948) at 1:700 dilution.
    Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L)(AS014) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection:ECL Basic Kit (RM00020).
    Exposuretime:60s.
    ABclonal:Immunofluorescence - ASAH1 Rabbit pAb (A13948)}

    Immunofluorescence - ASAH1 Rabbit pAb (A13948)

    Immunofluorescence analysis of SH-SY5Y cells using ASAH1 Rabbit pAb (A13948) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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    Secondary Antibodies (26)